摘 要: | 目的·研究不同月龄小鼠耳蜗中线粒体及烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide,NAD+)水平的变化,探索年龄相关性听力下降可能的机制。方法·分别选取1月龄、4月龄、8月龄及12月龄4组C57BL/6J雄性小鼠各10只,采用听性脑干反应(auditory brain response,ABR)和畸变产物耳声发射(distortion product otoacoustic emission,DPOAE)测定4组不同月龄小鼠听觉功能;通过实时荧光定量PCR (RT-qPCR)比较4组不同月龄小鼠耳蜗中线粒体能量代谢相关基因Ndufb5 (NADH:ubiquinone oxidoreductase subunit B5)、Sdha (succinate dehydrogenase complex flavoprotein subunit A)、 Sdhc (succinate dehydrogenase complex subunit C)和Atp5b (ATP synthase, H+transporting mito...
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