二级标准检测系统测定红细胞比积与参考方法检测结果的比较

目的探讨二级标准检测系统测定红细胞比积(Hct)的结果与参考方法测定结果的可比性。方法按照美国临床实验室标准化委员会(NCCLS)EP9-A2文件要求,以二级标准检测系统的检测方法作为被评价方法,依据NCCLS H7-A3文件,以微量比积离心测定法作为参考方法,选用40份健康人的新鲜血样本,同时使用前述的2种方法进行检测,对检测结果进行相关性和偏差评估。结果二级标准检测系统测定结果的偏差为0.45%~1.68%,相关系数为0.998 1。结论二级标准检测系统测定的Hct结果与参考方法测定结果的偏差较小,相关性良好,使用参考方法定值的新鲜血校准二级标准检测系统后,Hct测定结果与参考方法的结果具有良好的可比性。

Comparison of the secondary standard hematology analyzer-based method versus reference method

Objective To study the comparability of hematocrit(Hct) result between the secondary standard hematology analyzer-based method and the reference method. Methods According to the document of NCCLS EP9-A2,we took the secondary standard hematology analyzer-based method as test method and the reference method from the document H7-A3 of NCCLS as comparative method. 40 fresh blood specimens from healthy donors were selected as samples, and assayed by the above two methods at same time.Then we evaluated their correlation and bias. Results The bias of Hct value of secondary standard hematology analyzer-based method was 0.45%-1.68%.The Correlation coefficient between the test method and comparative method was 0.998 1. Conclusions There is a small bias and good comparability of Hct value between the secondary standard hematology analyzer-based method and the reference method after the secondary standard hematology analyzer is calibrated by the fresh blood measured by reference method.