“劳倦过度、房室不节”肾阳虚小鼠模型睾丸基因芯片研究

目的:应用基因芯片探讨劳倦过度、房室不节肾阳虚小鼠模型睾丸基因改变,从基因水平上探讨肾阳虚证和肾主生殖的现代科学机制。方法:连续4周采用雄雌鼠比例1:6同笼喂养和雄鼠每日游泳,建立劳倦过度、房室不节肾阳虚小鼠模型。用小鼠全基因组Oligo芯片检测对照组和模型组睾丸基因,以二者荧光信号相对强度比值≥2和≤0.5筛选差异表达基因,查阅北京博奥生物分子注释系统,进行基因功能分类。结果:成功建立劳倦过度、房室不节肾阳虚小鼠模型,绘制了对照组与模型组基因表达谱比较的散点图,筛选出差异表达基因,包括上调2425个,下调3080个。其中上调基因前百位中已知基因41个,下调基因前百位中已知基因62个,主要涉及细胞结构/功能、物质/能量代谢、信号转导/传递、炎症/免疫、细胞凋亡、转录/翻译、增殖/分化及细胞周期等。结论:肾阳虚小鼠睾丸基因发生广泛的改变,从基因水平对肾阳虚证的本质及肾主生殖的理论做出了一些诠释。

Study on testis gene chip of mouse model with syndrome of deficiency of kidney yang

Objective: To inquire into the testis gene change of mouse model with kidney-yang deficiency induced by overfatigue and excessive sexual life, the modern science mechanism of syndrome of kidney-yang deficiency and kidney controlling reproduction can be revealed on the gene level. Methods: The model of kidney-yang deficiency was established by each male mouse with six female mice keeping in the same cage, and all the male mice were forced to swim for 30-40 minutes everyday lasting for four weeks. The testis ...