| RP-HPLC法测定消炎利尿合剂中盐酸小檗碱的含量 |
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| 引用本文： | 卢荣枝,唐颂健,李运景,陈钧茂,张锦兴. RP-HPLC法测定消炎利尿合剂中盐酸小檗碱的含量[J]. 中国药房, 2008, 19(21): 1639-1640 |
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| 作者姓名： | 卢荣枝 唐颂健 李运景 陈钧茂 张锦兴 |
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| 作者单位： | 1. 广东中山市人民医院,中山市,528403
2. 华中科技大学同济医学院,武汉市,430030 |
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| 摘 要： | 目的:建立以RP-HPLC法测定消炎利尿合剂中盐酸小檗碱含量的方法。方法:色谱柱为Syltech-500C18(250mm×4.6mm,5μm),流动相为乙腈-0.02%磷酸二氢钾溶液(68:32),流速为1.0mL.min-1,检测波长为265nm,柱温为25℃。结果:盐酸小檗碱进样量在0.0272～0.2720μg范围内与峰面积积分值呈良好线性关系(r=0.9994);平均加样回收率为98.9%,RSD=1.06%(n=6)。结论:本方法简便、快速、准确,可用于消炎利尿合剂的质量控制。
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| 关 键 词： | 反相高效液相色谱法 盐酸小檗碱 消炎利尿合剂 含量测定 |
Determination of Berberine Hydrochloride in Xiaoyanliniao Mixture by RP - HPLC |
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| Affiliation: | LU Rong-zhi, LI Yun-jing,CHEN Jun-mao,ZHANC- Jin-xing,TANG Song-jian(1.Guangdong Zhongshan Municipal People＇ s Hospital,Zhongshan 528403, China;2.Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China) |
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| Abstract: | OBJECTIVE： To establish a RP HPLC method for the determination of Berberine hydrochloride in Xiaoyanliniao mixture. METHODS： The determination was performed using Syhech 500 C18 column（250 mm× 4.6 mm, 5μm） with column temperature at 25 ℃ . The mobile phase was acetonitrile 0.02% potassium dihydrogen phosphate （68 ： 32）with flow rate at 1.0 mL·min^-1. The dective wavelength was set at 265 nm. RESULTS： The linear range of Berberine hydrochloride was 0. 027 2 -0.272 0μg （ r = 0. 999 4）. The mean recovery was 98.0% （RSD = 1.06%. n = 6）. CONCLUSION： This method is simple, rapid, accurale and suitable for the quality control of Xiaoyanliniao mixture. |
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| Keywords: | RP HPI.C Berberine hydrochloride Xiaoyanliniao mixture： Content determination |
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