Nucleotide sequence andE. coli expression of the coat protein gene of the yellowing strain of soybean dwarf luteovirus

Summary The nucleotide sequence of the coat protein gene of the yellowing (Y) strain of soybean dwarf virus (SbDV) was determined from cloned cDNA. The gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated M_r of 22 200. The identity of the open reading frame (ORF) encoding the coat protein was confirmed by expression of anEscherichia coli -galactosidase fusion protein and detection by a dot blot immunoassay. Sequence comparisons of the deduced coat protein amino acids to several luteoviruses demonstrated that the SbDV-Y coat protein ORF shares greatest similarity with bean leaf roll virus (BLRV) (65%).