| 雄黄对多发性骨髓瘤细胞株RPMI8226细胞基因表达谱的作用 |
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| 引用本文： | 王孟昌,刘蓬勃. 雄黄对多发性骨髓瘤细胞株RPMI8226细胞基因表达谱的作用[J]. 医疗保健器具, 2010, 17(10): 1-3. DOI: 10.3936/j.issn.1674-4659.2010.10.001 |
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| 作者姓名： | 王孟昌 刘蓬勃 |
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| 作者单位： | 西安交通大学第一医院血液科,陕西,西安,710061 |
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| 摘 要： | 目的阐明雄黄治疗多发性骨髓瘤的分子机制。方法应用cDNA芯片在不同3天时间比较雄黄治疗前后72小时多发性骨髓瘤细胞系基因表达谱的改变。结果 cDNA芯片结果显示54条基因上调,60条基因下调,进一步分析发现17条上调基因Z-score大于2,3条下调基因Z-score小于-2。结论这些基因是本研究中雄黄治疗后显著改变的基因,CCL2,CCL3,BTG1,TNFAIP3,TNFAIP8,SLC38A2,IGFBP4是重要的上调基因,涉及一系列的细胞生命活动如：细胞增殖、细胞间信号转导、凋亡调节及细胞内环境稳定。有3个显著下调的基因涉及到肌肉收缩。部分基因（CCL2,TNFAIP3,BTG1）的结果通过反转录聚合酶链反应证实。
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| 关 键 词： | 雄黄 基因芯片 多发性骨髓瘤 RPMI8226细胞 |
Gene Expression Profile of Multiple Myeloma Cell Line Treated by Realgar |
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| WANG Mengchang,LIU Pengbo. Gene Expression Profile of Multiple Myeloma Cell Line Treated by Realgar[J]. Medicine Healthcare Apparatus, 2010, 17(10): 1-3. DOI: 10.3936/j.issn.1674-4659.2010.10.001 |
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| Authors: | WANG Mengchang LIU Pengbo |
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| Affiliation: | WANG Mengchang,LIU Pengbo(Department of Hematology,the First Hospital of Xi'an Jiaotong University,Xi'an 710061,China) |
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| Abstract: | Objective In order to elucidate the molecular mechanism of realgar treatment for multiple myeloma(MM) . Methods cDNA microarray was used to compare the gene expression profile of MM cell line RPMI8226 at 72 hrs pre-and post-realgar treatment on three separate days. Results 54 up-regulated and 60 down-regulated genes were identified by cDNA microarray,further analysis screened out 17 up-regulated and 3 down-regulated genes with Z-score greater than 2 or less than-2. Conclusion These genes can be considered t... |
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| Keywords: | Realgar cDNA microarray Multiple myeloma RPMI8226 cells |
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