| 帕金森病小鼠黑质多巴胺能神经元凋亡诱导因子核移位的研究 |
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| 引用本文： | 王东玉,张朝东.帕金森病小鼠黑质多巴胺能神经元凋亡诱导因子核移位的研究[J].解剖科学进展,2009,15(1):56-59. |
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| 作者姓名： | 王东玉 张朝东 |
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| 作者单位： | 1. 锦州市中心医院神经内科,辽宁,锦州,121000
2. 中国医科大学第一临床学院神经内科,辽宁,沈阳,110001 |
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| 摘 要： | 目的探讨帕金森病(PD)小鼠模型黑质凋亡诱导因子(AIF)的核移位情况及其与多巴胺(DA)能神经元损伤之间的关系。方法采用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)制备PD小鼠模型,2h、24h、72h后取中脑组织进行酪氨酸羟化酶免疫染色观察黑质DA能神经元的损害情况,AIF免疫组化染色和Western blot方法检测AIF的核移位情况。结果与对照组比较,PD组小鼠黑质DA能神经元数量随时间呈递减趋势,并出现AIF核移位,2h时达高峰,之后随时间呈下降趋势。结论AIF核移位是PD小鼠黑质DA能神经元损伤的早期指标,在PD的发病过程中发挥重要作用。
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| 关 键 词： | 帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 凋亡诱导因子 |
Involvement of Mitochondrio-nuclear Translocation of Apoptosis-inducing Factor in Dopaminergic Neurons of Substantia Nigra in Mouse Model of Parkinson's Disease |
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| WANG Dong-yu,ZHANG Chao-dong.Involvement of Mitochondrio-nuclear Translocation of Apoptosis-inducing Factor in Dopaminergic Neurons of Substantia Nigra in Mouse Model of Parkinson's Disease[J].Progress of Anatomical Sciences,2009,15(1):56-59. |
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| Authors: | WANG Dong-yu ZHANG Chao-dong |
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| Institution: | 1.Department of Neurology;Central Hospital of Jinzhou;Liaoning Jinzhou 121000;2.Department of Neurology;the First Affiliated Hospital of China Medical University;Liaoning Shenyang 110001 China |
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| Abstract: | Objective To study the relationship of apoptosis-inducing factor(AIF) translocation and dopaminergic neurons damage in substantia nigra of Parkinson' s disease(PD) model mice.Methods 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced PD model was performed in mice.The midbrains were removed at 2h,24h and 72h time points to observe the destruction of dopaminergic neurons by tyrosine hydroxylase(TH) immunohistochemical analysis.AIF translocation was observed by immunohistochemistry and Western blot.Re... |
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| Keywords: | Parkinson's disease MPTP apoptosis-inducing factor |
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