| 青蒿琥酯对脂多糖诱导的BV-2小胶质细胞激活的抑制作用 |
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| 引用本文： | 史俊,;王巧燕,;王敦敬,;刘永海,;肖成华.青蒿琥酯对脂多糖诱导的BV-2小胶质细胞激活的抑制作用[J].徐州医学院学报,2014(6):388-390. |
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| 作者姓名： | 史俊 ;王巧燕 ;王敦敬 ;刘永海 ;肖成华 |
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| 作者单位： | [1]徐州医学院研究生学院2011级,江苏徐州221004; [2]徐州医学院附属医院神经内科,江苏徐州221002 |
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| 基金项目： | 徐州医学院研究生科研创新计划（XYYC-1212） |
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| 摘 要： | 目的 观察青蒿琥酯对脂多糖（LPS）诱导的BV-2小胶质细胞炎症反应的抑制作用.方法 LPS诱导BV-2小胶质细胞活化建立炎症模型,观察青蒿琥酯（0.5、1.5、3.5 μmol/L）对细胞的作用.MTT检测细胞活性;Griess试剂法检测一氧化氮（NO）的释放;Western Blot检测核转录因子κB（NF-κB）的蛋白表达.结果 青蒿琥酯减少了活化的BV-2小胶质细胞NO的释放,降低了核内NF-κB的蛋白表达.结论 青蒿琥酯可能通过抑制LPS诱导的BV-2小胶质细胞NF-κB的激活从而抑制NO的产生,发挥抗炎作用。
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| 关 键 词： | 青蒿琥酯 脂多糖 BV-2小胶质细胞 一氧化氮 核转录因子κB |
Artesunate inhibits the activation of BV -2 microglial cells induced by lipopolysaccharide |
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| Institution: | SHI Jun, WANG Qiaoyan , WANG Dunjing, LIU Yonghai, XIAO Chenghua ( 1. Grade 2011, Graduate School, Xuzhou Medical College, Xuzhou, Jiangsu 221004, China; 2. Department of Neurology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221002) |
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| Abstract: | Objective To investigate the inhibitory effects of artesunate on the activation of BV2 microglia cells induced by lipopolysaccharide （LPS）.Methods An inflammatory model was established where LPS induced the activation of BV-2 microglial cells,and the effect of artesunate （0.5,1.5 and 3.5 μmol/L） on the cells was observed.Cell viability was detected by MTT assay.The release of nitric oxide （NO） was detected by Griess reagent.The expression of nuclear NF-κB was detected by Western blot.Results Artesunate reduced the release of NO and the expression of nuclear NF-κB in activated BV2 microglia cells.Conclusion Artesunate may take an anti-inflammatory role through inactivating NF-κB and reducing the amount of NO in LPS-induced BV2 microglia cells. |
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| Keywords: | artesunate lipopolysaccharide BV-2 microglial cells nitric oxide NF-κB |
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