| 黄芪液拮抗乳酸盐腹透液对人腹膜间皮细胞的损伤作用 |
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| 引用本文： | 刘映红,刘伏友,段绍斌,彭佑铭,肖平,李军,卓莉,袁芳,樊敏.黄芪液拮抗乳酸盐腹透液对人腹膜间皮细胞的损伤作用[J].中国现代医学杂志,2002,12(16):32-33,39. |
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| 作者姓名： | 刘映红 刘伏友 段绍斌 彭佑铭 肖平 李军 卓莉 袁芳 樊敏 |
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| 作者单位： | 中南大学湘雅二医院肾科,长沙,410011 |
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| 基金项目： | 湖南省自然科学基金项目 (0 1JJY2 0 71)，湖南省卫生厅基金项目 (2 0 0 1-Y -3 9) |
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| 摘 要： | 目的 :研究黄芪液对乳酸盐腹透液致腹膜间皮细胞增殖及损伤作用的影响。方法 :采用胰蛋白酶 -ED TA消化法 ,从人腹膜组织中分离间皮细胞 ,并建立体外人腹膜间皮细胞 (HPMC)培养模型。以MTT法测定HPMC增殖程度 ,采用自动生化分析仪检测 ,培养液中乳酸脱氢酶 (LDH)水平示细胞损伤程度。结果 :2 .5 %乳酸盐腹透液中加用黄芪液 (1mg/ml)与HPMC作用 4 5min ,其培养液中LDH水平较腹透液对照组明显降低(P <0 .0 1) ,HPMC增殖能力明显增强 (P <0 .0 1)。结论 :黄芪液能拮抗乳酸盐腹透液致HPMC的损伤。
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| 关 键 词： | 腹膜透析 间皮细胞 腹膜 乳酸盐类 黄芪注射液 |
EFFECTS OF HUANGQI INJECTION ON THE CYTOTOXICITY OF LACTATE-BUFFERED PERITONEAL DIALYSIS SOLUTES TO HUMAN PERITONEAL MESOTHELIAL CELLS |
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| Liu Yinghong,Liu Fuyou,Duan Shaobin,et al..EFFECTS OF HUANGQI INJECTION ON THE CYTOTOXICITY OF LACTATE-BUFFERED PERITONEAL DIALYSIS SOLUTES TO HUMAN PERITONEAL MESOTHELIAL CELLS[J].China Journal of Modern Medicine,2002,12(16):32-33,39. |
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| Authors: | Liu Yinghong Liu Fuyou Duan Shaobin |
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| Institution: | Liu Yinghong,Liu Fuyou,Duan Shaobin,et al. Department of Nephrology,The Second Xiangya Hospital,Central South University,Changsha 410011$$$$ |
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| Abstract: | Objective:To determine the effects of huangqi injection on the cytotoxicity of lactate-buffered peritoneal dialysis solutes(L-PDS) to human peritoneal mesothelial cells (HPMC).Methods:HPMC was separated with trypsin-EDTA.HPMC proliferation and the concentration of lactate dehydrogenase (LDH) in supernatants were detected by MTT assay and automatic biochemical analyzor,respectively.Results:The addition of 1mg/ml of huangqi to 2.5% L-PDS caused an increase in HPMC proliferation, and a decrease in release of LDH for exposure 45min( P <0.01).Conclusion:Huangqi injection can reverse the injury of L-PDS to HPMC. |
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| Keywords: | Peritoneal Dialysis Mesothelial Cells Peritoneal Lactates Huangqi injection |
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