首页 | 本学科首页   官方微博 | 高级检索  
     

桂皮酸诱导K562细胞凋亡及其机制的初步研究
引用本文:孙海燕,王季石,李栋博,方琴,李辰蕊. 桂皮酸诱导K562细胞凋亡及其机制的初步研究[J]. 贵州医药, 2006, 30(2): 112-113
作者姓名:孙海燕  王季石  李栋博  方琴  李辰蕊
作者单位:贵阳医学院附属医院血液科,550004
摘    要:目的探讨桂皮酸对K562细胞凋亡的诱导作用及其机制。方法应用MTT测出 IC50,应用免疫荧光显微镜,DNA Ladder,流式细胞仪,检测凋亡的发生,应用RT-PCR琼脂糖凝胶电泳检测bcl-2基因表达的差异。结果经MTT检测发现IC50为1 mmol/L,24 h后药物基本达到了较佳的效果,且免疫荧光显微镜、DNA Ladder、流式细胞仪检测均有凋亡的发生,RT-PCR结果显示,经桂皮酸处理24h后,bcl-2基因表达明显降低。结论1 mmol/L的桂皮酸作用24 h后可以引起K562 细胞发生凋亡,并且bcl-2基因表达下调是引起凋亡的原因之一。

关 键 词:细胞凋亡  K562细胞  桂皮酸
文章编号:1000-744X(2006)02-0112-02

Study of K562 of the apoptosis induction by cinnamic aicd and the mechanism
Su Haiyan ,Wang Jishi , Li Dongbo ,et al.. Study of K562 of the apoptosis induction by cinnamic aicd and the mechanism[J]. Guizhou Medical Journal, 2006, 30(2): 112-113
Authors:Su Haiyan   Wang Jishi    Li Dongbo   et al.
Affiliation:Department of hematology, the Affiliated Hospital of Guiyang Medical College ,Guiyang 550004
Abstract:Objective To investigate K562 apoptosis induction by cinnamic acid as well as the related action of bcl-2 gene. Methods Divide K562 cells into 2 groups: the cinnamic acid was added to group A, group B was as control group. The K562 cells in two groups were tested by MTT, fluorescence microscopy, flow cytometry, RT-PCR, electrophoresis. Results poptosis was detected in group A by fluorescence microscopy and electrophoresis respectively. The expression of bcl-2 was statistically significant between two groups tested by RT-PCR, B>A (P<0. 05). Conclusion The results of this investigation showed cinnamic acid may induce the apoptosis of K562 cells by downregulation of bcl-2 expression.
Keywords:Apoptosis K562 Bcl-2 gene
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号