蛋白质组研究中双向聚丙烯酰胺凝胶电泳图谱的计算机分析

目的：建立蛋白质组研究中双向聚丙烯酰胺凝胶电泳（ｔｗｏ－ｄｉｍｅｎｓｉｏｎａｌｐｏｌｙａｃｒｙｌａｍｉｄｅｇｅｌｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ，２－ＤＰＡＧＥ）图谱的计算机分析方法。方法：利用ＩｍａｇｅＭａｓｔｅｒ２－Ｄ分析软件。结果：我们对人肝癌细胞株ＨｅｐＧ２蛋白质组的２－ＤＰＡＧＥ图谱进行了计算机分析，识别了１０００以上的蛋白斑点（其中肉眼可视斑点为４００左右），同时获得了所识别斑点的等电点、相对分子质量、斑点面积、Ｄ值、Ｄ％、斑点体积及相对体积等参数。结论：建立了蛋白质组研究中图像分析体系，为今后蛋白质组２－ＤＰＡＧＥ数据的分析与比较、数据库的建立、蛋白质的功能预测以及大规模斑点的模式识别奠定了基础。

Computer analysis of two dimensional polyacrylamide gel electrophoresis images in proteome research

Objective: To establish a method for computer analysis of two dimensional polyacrylamide gel electrophoresis(2 D PAGE) image in proteome research. Methods: Using ImageMaster 2 D computer software. Results: In proteome research,2 DE images of proteins of human hepatic carcinoma cell line HepG2 were processed using ImageMaster computer software. More than 1 000 protein spots were detected, and their isoelectric point (pI), molecular mass , D value, D%, Vvalue and V% data were obtained. Conclusion: We have established computer image analysis system for proteome research which is the basis of future research on analysis and comparison of proteome 2 D PAGE data, establishment of proteome 2 DE database, forecast of the functions of proteins and large scale model recognition of protein spots.