| 山东半岛地区贝类中诺如病毒污染状况与病毒鉴定初报 |
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| 引用本文： | 苏来金,马丽萍,徐仰丽,徐静,周德庆. 山东半岛地区贝类中诺如病毒污染状况与病毒鉴定初报[J]. 中国人兽共患病杂志, 2010, 26(5): 451-454 |
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| 作者姓名： | 苏来金 马丽萍 徐仰丽 徐静 周德庆 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所;温州市农业科学研究院食品所; |
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| 基金项目： | 公益性行业(农业)科研专项 |
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| 摘 要： | 目的调查研究山东半岛沿海地区双壳贝类中诺如病毒污状况并对阳性株进行鉴定。方法应用常规RT-PCR与实时荧光定量RT-PCR,对2007年9月到2008年8月间在山东半岛沿海8个地区收集的186份双壳贝类样品进行了诺如病毒的定性和定量检测,并对阳性株核酸片段进行克隆、转化和序列分析,使用DNAStar软件进行核酸序列同源性比较,经MEGA4绘制系统进化树。结果 186份样品中检出5份诺如病毒阳性,检出率为2.67%,诺如病毒含量为大于102拷贝RNA,序列分析与遗传进化树显示5份诺如病毒阳性株均为GGII型NVs,与国内报道的GGII型毒株同源性达到98%以上。结论山东半岛地区贝类中含有的诺如病毒以GGII型为主,冬季检出率较高。
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| 关 键 词： | 贝类 诺如病毒 污染状况 |
| 收稿时间： | 2010-05-20 |
Noroviruses pollution status of shellfish in the area of Shandong Peninsula and preliminary identification of virus |
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| SU Lai-jin,MA Li-ping,XU Yang-li,XU Jing,ZHOU De-qing. Noroviruses pollution status of shellfish in the area of Shandong Peninsula and preliminary identification of virus[J]. Chinese Journal of Zoonoses, 2010, 26(5): 451-454 |
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| Authors: | SU Lai-jin MA Li-ping XU Yang-li XU Jing ZHOU De-qing |
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| Affiliation: | SU Lai-jin,MA Li-ping,XU Yang-li,XU Jing,ZHOU De-qing(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China) |
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| Abstract: | This study aimed to investigate the Noroviruses (NVs) pollution of shellfish in the area of Shandong Peninsula and to determine the genotypes of the positive isolated strains. 186 shellfish samples collected from eight different areas between September 2007 and August 2008 were detected by routine RT-PCR and Real-time RT-PCR respectively. The nucleic acid fragment of positive isolated strains were cloned,seguenced and blasted with GenBank. The nucleotide sequence homology was also evaluated with DNAStar pac... |
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| Keywords: | shellfish noroviruses pollution status positive strains |
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