维持性血液透析患者输血传播性病毒感染状况及其基因分析

目的研究维持性血液透析患者输血传播性病毒(TTV)感染状况,及进行其部分基因序列分析.方法设计TTVORF1部分基因引物,巢式PCR检测80例血液透析患者及12例血液透析工作人员血清标本,将PCR产物克隆、测序分析.结果血液透析患者TTV感染率为35%(28/80),12名工作人员中有1例呈TTV阳性(8.3%).与日本株相比较,本实验分离株TTVORF1部分基因同源性为96.7%.TTV合并HBV感染者8例(8%),合并HCV感染者5例(6%);TTV与HBV、HCV同时感染者2例(2%).TTV阳性患者中,有输血史者占57%.结论血液透析患者中存在严重的TTV感染,血液透析工作人员亦存在TTV感染.TTV可以与HBV、HCV重叠感染,TTV感染者可能与输血有关,但TTV的非血液传播同样应引起重视.

Transfusion transmitted virus infection and its genetic analysis in maintenance hemodialysis patients

Objective To investigate the infection of transfusion transmitted virus(TTV) in maintenance hemodialysis patients and analyse a part of TTV DNA sequence. Method Denome DNA of TTV ORF1 was amplified in sera from 80 hemodialysis patients and 12 nurses of dialysis center using a nested polymerase chain reaction(PCR) assay with specific primers. After molecular cloning and sequencing of the PCR products, the genetic valuations of the TTV DNA were analyzed. Results 35 % (28/80)of hemodialysis patients and Eight. 3% (1/12) of dialysis operators were TTV DNA postitive. Compared with the reported Japanese isolate, the mucleotide sequence homologies of TFV isolates in this experiment was 96.7%. 8 patients had HBV and TTV infection, 5 patients HCV and TTV infection, while 2 patients had HBV, HCV and TTV infection. 57% of the TTV DNA positive patients had the transfusion history. Conclusions TTV infection is seriously in hemodialysis patients, of whom most have transfusion history, and can be found in dialysis operators. TTV can be co-infected with HBV and HCV. The infection of TTV without transfusion should be considered as well.