枳壳挥发油提取工艺优选及其化学成分GC-MS分析

目的:优选水蒸气蒸馏法提取枳壳挥发油的工艺,分析粉碎度对枳壳挥发油化学成分的影响。方法:采用正交试验确定水蒸气蒸馏法提取枳壳挥发油的工艺条件,以挥发油提取率为评价指标,粉碎度、加水量、浸泡时间、提取时间为考察因素。采用GC-MS对枳壳粉碎度为24目和50目所提取的挥发油进行化学成分鉴定。结果:最佳水蒸气蒸馏法工艺条件为枳壳粉碎度24目,加11倍量水浸泡0.5 h,提取7 h。从24目枳壳粉末挥发油中鉴定出45个组分,50目中鉴定出39个组分,分别占其挥发油总量的99.46%和99.53%。结论:优化的提取工艺重复性好、挥发油提取率较高且成分种类多,适用于枳壳挥发油的提取。

Optimization of Extraction Process of Volatile Oil from Aurantii Fructus and GC-MS Analysis of Its Chemical Constituents

Objective: To optimize extraction process of volatile oil from Aurantii Fructus,and analyze difference of chemical constituents in volatile oil from Aurantii Fructus with grinding degree of 24 mesh and 50 mesh. Method: Volatile oil from Aurantii Fructus was extracted by steam distillation,orthogonal test was adopted to optimize process of volatile oil.GC-MS was employed to compare similarities and differences between volatile oil from Aurantii Fructus with grinding degree of 24 mesh and 50 mesh. Result: Optimum extraction conditions of steam distillation were as follows:the comminution degree of 24 mesh,soaked 0.5 h and extracted 7 h with 11 times the amount of water.Forty-five components in volatile oil from Aurantii Fructus with grinding degree of 24 mesh were identified,there were 39 components for 50 mesh,accounting for 99.46%and 99.53%of total content of volatile oil,respectively. Conclusion: This optimized process is stable and feasible with high extraction rate of volatile oil.