阿司匹林对大鼠脊髓损伤细胞凋亡的保护作用

目的 观察阿司匹林对大鼠慢性压迫性脊髓损伤后神经细胞凋亡及神经功能恢复的影响。方法选择65只体重为220～250g的Wistar大鼠（雌雄不限），于T10部位置入后路渐进式压迫装置，制作成慢性压迫性脊髓损伤模型。随机分为阿司匹林治疗组（A组，30只）、生理盐水对照组（B组，30只）和假手术组（C组，5只）。应用原位末端脱氧核糖核苷酸转移酶介导dUTP标记技术，分别于慢性压迫性脊髓损伤后1、3、7、14、28d做行为学评价，并取材对脊髓损伤区进行细胞凋亡检测。结果A、B组均发现细胞凋亡，A组与B组细胞凋亡率相比差异有显著性（P〈0．05），A组与B组行为学评价相比差异有显著性（P〈0．05），神经细胞凋亡情况与运动功能改变具有相关性。结论 阿司匹林对慢性脊髓压迫损伤后所导致的神经细胞凋亡产生抑制作用。

Neuroprotection Effect of Aspirin on Neuron Apoptosis Following Chronic Spinal Cord Compressive Injury in Rats

Objective This study evaluated the neuroprotection effect of aspirin on neuron apoptosis following chronic spinal cord compressive injury in rats. Methods The chronic spinal cord compression model made of 65 Wistar mice（Weight 220-250 g） were assigned into the following 4 groups randomly. 30 mice into the aspirin therapy group （Group A）,30 mice into the normal sodium （NS） control group （Group B） and 5 mice into the sham operation group （Group C）. Applied the locomotor＇s function scale and the TUNEL immunohistochemistry technique labeled of the spinal cord injuring profiles on 1st, 3rd, 7th, 14th, 28th days of post operation respectively. Result Group A reduced the neuron apoptosis,the Apoptosis Index （AI） and the results of locomotor＇s function scale compare with Group B,those that has significant difference （P〈0.05）. There is a high relativity between the neuron apoptosis and locomotor＇s function. Conclusion These results suggest that the protective effects of aspirin against neuron apoptosis following chronic spinal cord compressive injury.