茶多酚对大鼠血管平滑肌细胞增殖的影响

目的　探讨茶多酚对离体大鼠胸主动脉血管平滑肌细胞增殖的影响。方法　体外培养大鼠主动脉血管平滑肌细胞 ,应用不同剂量的茶多酚作用 2 4 h后 ,采用 MTS/ PES法确定血管平滑肌细胞的增殖状态。应用流式细胞术测定细胞周期。结果　与对照组相比 ,茶多酚对血管平滑肌细胞增殖具有明显的抑制作用 (P<0 .0 0 1)。流式细胞术检测结果表明 ,茶多酚可以使血管平滑肌细胞大部分处于 G0 / G1 期 ,与对照组相比有明显差异 (P<0 .0 5 ) ,并且可以诱导其凋亡。结论　茶多酚可明显抑制离体大鼠血管平滑肌细胞的增殖。

Effects of tea polyphenols on rat vascular smooth muscle cells proliferation

Object The purpose of this study was to determine the effects of tea polyphenols on rat vascular smooth muscle cells (VSMCs) proliferation. Methods Rat aortic VSMCs were cultured and treated with tea polyphenols for 24 h. Cell proliferation was quantified by colormetric assay and definited by MTS/PES. Cell cycle analysis was performed by flow cytometry. Results Compared with the control group, VSMCs growth was significantly inhibited by tea polyphenols (P<0.001). Cell number in G 0/G 1 phase of tea polyphenols (1 mg/mL) group was higher than those of the control group (P<0.05) by flow cytometry analysis. Apoptosis was observed in tea polyphenols groups by flow cytometry. Conclusion The tea polyphenols can markedly inhibit the rat VSMCs proliferation.