| 血清乙肝病毒DNA的快速提取及定量 PCR测定中应用的探讨 |
| |
| 引用本文： | 罗心静,徐克前. 血清乙肝病毒DNA的快速提取及定量 PCR测定中应用的探讨[J]. 中国现代医学杂志, 2004, 14(18): 124-126 |
| |
| 作者姓名： | 罗心静 徐克前 |
| |
| 作者单位： | 1. 台州学院医学院,检验系,浙江,台州,318000
2. 中南大学湘雅医学院,检验系,湖南,长沙,410078 |
| |
| 摘 要： | 目的寻找简单、快速地提取血清乙型肝炎病毒(HBV)DNA并能应用于定量PCP,测定的方法.方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清HBV DNA,比较荧光定量PCP测定的重复性和测定值的差异.结果碱裂解法所得HBV DNA量高于酚氯仿法和煮沸裂解法(P＜0.05),并且碱裂解法所得结果的重复性好于酚氯仿法和煮沸裂解法(CV分别为0.21,0.28和0.33).结论碱裂解法提取HBV DNA简单、快速,适用于HBV DNA荧光定量测定.
|
| 关 键 词： | 病毒 脱氧核糖核酸 提取 聚合酶链反应 |
| 文章编号： | 1005-8982(2004)18-0124-03 |
Rapid method for hepatitis B virus DNA extraction from serum for quantitative PCR amplification |
| |
| LUO Xin-jing,XU Ke-qian. Rapid method for hepatitis B virus DNA extraction from serum for quantitative PCR amplification[J]. China Journal of Modern Medicine, 2004, 14(18): 124-126 |
| |
| Authors: | LUO Xin-jing XU Ke-qian |
| |
| Abstract: | Objective: To develop a simple and rapid method for extracting nucleic acid from serum on hepatitis B virus (HBV) DNA detection by polymerase chain reaction (PCR). Methods: An alkaline lysis method was used to isolate serum HBV DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method. Results: The HBV DNA quantitative values by PCR using the alkaline lysis were higher than the phenol-chloroform extraction method and boiling lysis method (P <0.05). Morever, the HBV DNA quantitative values by PCR using the alkaline lysis were more reproducible than the phenol-chloroform extraction method and boiling lysis method(CV:0.21, 0.28 and 0.33, respectively). Conlusions: The alkaline lysis method is simple, rapid and efficient method for extraction of serum HBV DNA and suitable for HBV DNA quantitative PCR detection. |
| |
| Keywords: | viral DNA extraction PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
