| 利用BgAMA-1抗原建立吉布森巴贝斯虫的ELISA诊断方法 |
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| 引用本文： | 杨俊,周金林,肖兵南.利用BgAMA-1抗原建立吉布森巴贝斯虫的ELISA诊断方法[J].中国寄生虫学与寄生虫病杂志,2006,24(Z1):62-64. |
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| 作者姓名： | 杨俊 周金林 肖兵南 |
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| 作者单位： | 湖南省畜牧兽医研究所,长沙,410131 |
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| 摘 要： | 本研究利用基因重组技术,构建并表达吉布森巴贝斯虫的重组顶膜抗原-1(BgAMA-1),将BgAMA-1作为诊断抗原,建立针对吉布森巴贝斯虫的ELISA诊断方法。结果显示,构建的重组表达质粒得以表达,建立的ELISA方法在血清学诊断中有较好的特异性。
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| 关 键 词： | 吉布森巴贝斯虫 顶膜抗原-1 ELISA |
| 文章编号： | 1000-7423(2006)-Supp1-0062-03 |
| 修稿时间： | 2006年11月10 |
The Establishment of ELISA Method for Detecting Babesia gibsoni by Recombinant Antigen BgAMA-1 |
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| YANG Jun,ZHOU Jin-lin,XIAO Bin-nan.The Establishment of ELISA Method for Detecting Babesia gibsoni by Recombinant Antigen BgAMA-1[J].Chinese Journal of Parasitology and Parasitic Diseases,2006,24(Z1):62-64. |
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| Authors: | YANG Jun ZHOU Jin-lin XIAO Bin-nan |
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| Abstract: | In order to improve diagnostic method to Babesia gibsoni infection,gene recombination technique was used in our study.By this technique,we constructed and expressed recombinant apical membrane antigen-1(BgAMA-1),and recombinant BgAMA-1 was used in ELISA experiment as diagnostic antigen.The results showed the recombinant expressed plasmid was triumphantly constructed and the recombinant antigen BgAMA-1 had a high specificity in ELISA test.These results demonstrate that the recombinant BgAMA-1 might be a useful diagnostic reagent for detection of antibodies to B.gibsoni in dogs. |
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| Keywords: | Babesia gibsoni BgAMA-1 ELISA |
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