| 当归芍药散入血成分的分析及药代动力学研究(英文) |
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| 引用本文： | 陈林霖,王艳慧,戚进,董婷霞,朱丹妮,余伯阳. 当归芍药散入血成分的分析及药代动力学研究(英文)[J]. 中国天然药物, 2011, 0(5): 363-368 |
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| 作者姓名： | 陈林霖 王艳慧 戚进 董婷霞 朱丹妮 余伯阳 |
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| 作者单位： | 中国药科大学中药复方研究室;香港科技大学中医药研究中心;现代中药教育部重点实验室(中国药科大学); |
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| 基金项目： | supported by the National Natural Science Foundation of China (No. 30772792); a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions~~ |
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| 摘 要： | 目的:研究大鼠口服给药当归芍药散后体内的入血成分及其药代动力学。方法:大鼠灌胃给予当归芍药散提取物后,以HPLC-DAD-MSn分析含药血清中药物成分,并建立主要入血成分的HPLC测定方法以应用于药代动力学研究。结果:大鼠灌胃给予当归芍药散提取物后血清中检测到7个原型成分和1个代谢产物,并对大鼠血浆中芍药内酯苷、芍药苷、阿魏酸和藁本内酯进行了测定。方法学考察了线性范围(0.025-5.60μg·ml-1)、定量限(0.022-0.18μg·ml-1)、日内和日间精密度(<10.1%)、准确度(92.5%-111.5%)和回收率(>80%)。结论:芍药苷硫酸酯、芍药内酯苷、芍药苷、阿魏酸、川芎内酯I、藁本内酯和丁烯基苯酞为当归芍药散主要的入血成分。对大鼠血浆中芍药内酯苷、芍药苷、阿魏酸和藁本内酯建立的测定方法简便、准确、灵敏度高,适用于血药浓度测定及其药动学研究。
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| 关 键 词： | 当归芍药散 血清药物化学 药代动力学 |
Identification and Determination of Absorbed Components of Danggui-Shaoyao-San in Rat Plasma |
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| CHEN Lin-Lin,WANG Yan-Hui,QI Jin,DONG Ting-Xia,ZHU Dan-Ni,YU Bo-Yang. Identification and Determination of Absorbed Components of Danggui-Shaoyao-San in Rat Plasma[J]. Chinese JOurnal of Natural Medicines, 2011, 0(5): 363-368 |
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| Authors: | CHEN Lin-Lin WANG Yan-Hui QI Jin DONG Ting-Xia ZHU Dan-Ni YU Bo-Yang |
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| Abstract: | AIM:To investigate the absorbed components of Danggui-Shaoyao-San(DSS) in rat plasma and their pharmacokinetics.METHODS:Serum samples from rats with DSS extract administrated orally were analyzed by HPLC-DAD-MSn and an HPLC-DAD method was established for the determination.RESULTS:Seven prototypes and one metabolite were identified in rat serum,and albiflorin,paeoniflorin,ferulic acid and ligustilide in rat plasma were determined for pharmacokinetic study.The proposed method was validated with a linear range... |
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| Keywords: | Danggui-Shaoyao-San Serum pharmacochemistry Pharmacokinetics |
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