| 分光光度法测定红树秋茄中总黄酮含量 |
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| 引用本文： | 计燕萍,唐岚. 分光光度法测定红树秋茄中总黄酮含量[J]. 北方药学, 2016, 13(4): 5-6 |
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| 作者姓名： | 计燕萍 唐岚 |
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| 作者单位： | 嘉兴学院 嘉兴 314001;浙江工业大学 杭州 310014 |
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| 摘 要： | 目的：建立红树秋茄中总黄酮含量的测定方法。方法：采用紫外分光光度法，通过单因素试验优选显色方法和显色条件，建立总黄酮含量的测定方法。结果：采用NaNO2-Al（NO3）3-NaOH法，以芦丁作为对照品，在0.02～0.1mg/mL浓度范围内线性关系良好， r2=0.9999，平均回收率为103.02%（RSD=2.2%.n=8）。结论：该方法准确、灵敏、重现性好，可用于秋茄总黄酮含量的测定。
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| 关 键 词： | 秋茄总黄酮 紫外分光光度法 含量测定 |
Spectrophotometric Determination of Total Flavonoids in Kandelia candel |
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| Ji Yanping,Tang Lan. Spectrophotometric Determination of Total Flavonoids in Kandelia candel[J]. BEIFANG YAOXUE, 2016, 13(4): 5-6 |
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| Authors: | Ji Yanping Tang Lan |
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| Affiliation: | Ji Yanping;Tang Lan;Jiaxing University;Zhejiang University of Technology; |
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| Abstract: | Objective: To establish a method for determination of the content of total flavonoids in Kandelia candel.Method: single factor tests were adopted to optimize colorimetry methods and conditions by UV FDS,determination of the content of total flavonoids was established. Result: Selecting rutin as a reference substance,NaNO2-Al (NO3)3-NaOH method was adopted,rutin had a liner relationship with absorbance in the range of 0.02~0.1mg/mL,r2=0.9999,average recovery was 103.02% with RDS of 2.2%,n=8. Conclusion: NaNO2-Al (NO3)3-NaOH method appeared to be an accurate,reliable and repeatable system fordetermination of total flavonoids in Kandelia candel. |
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| Keywords: | Kandelia candel Total flavonoids Ultraviolet spectrophotometry Determination |
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