TGF-β1和p21WAF1在中耳胆脂瘤中的表达及其意义

［收稿日期］ ［基金项目］ ［作者简介］ 刘继丽（1980-）,女，吉林省龙井市人，医学硕士，主要从事中耳胆脂瘤的研究。 目的： 探讨TGF-β1和p21WAF1在中耳胆脂瘤中的表达，了解两者在胆脂瘤发病中的作用。方法： 应用免疫组化技术检测20例中耳胆脂瘤组和10例正常对照组中TGF-β1和p21WAF1的表达。结果： ①p21WAF1定位于细胞核，呈棕黄色颗粒状；TGF-β1主要胞浆着色，呈棕黄色颗粒状。②中耳胆脂瘤组p21WAF1阳性表达率65％，分布于上皮的全层，以靠近基底层的棘细胞层和颗粒层显著，阳性指数（LI）均值为28.9%±6.7%；而正常对照组未见p21WAF1阳性表达。p21WAF1在中耳胆脂瘤上皮中的表达高于对照组（P<0.05）。③中耳胆脂瘤组TGF-β1阳性表达率90％， 位于上皮全层， LI为 32.3%±5.7%；在正常外耳道皮肤中阳性表达率为50％，LI 为13.3%±4.9%，两组比较差异有显著性（P<0.05）。④在中 耳胆脂瘤组p21WAF1与TGF-β1表达LI均值比较差异无显著性（P>0.05），p21WAF1表达LI均值与TGF-β1表达LI均值呈正相关关系（r＝0.913，P<0.01）。结论：TGF-β1和p21WAF1蛋白促进胆脂瘤发生发展，并具有相关性。 ［关键词］ 转化生长因子β;p21WAF1;中耳胆脂瘤;细胞凋亡 ［中图分类号］ ［文献标识码］ A  （）

Expressions of TGF-β1 and p21WAF1 in middle ear cholesteatoma and their significances

Objective To study the expressions of TGF-β1 and p21WAF1 in middle ear cholesteatoma and discuses their effects on cholesteatoma.Methods The expressions of TGF-β1 and p21WAF1 were determined by immunohistochemical S-P technology and computer image analysis in 20 specimens of cholesteatoma epithelium and 10 specimens of normal external canal skin.Results ① p21WAF1 and TGF-β1 could be detected in middle ear cholesteatoma and in normal ear canal skin.p21WAF1 was located at karyon,taking on yellow-brown pellet;TGF-β1 was located at cytoplasmic,taking on yellow-brown pellet.②The p21WAF1 positive rate in middle ear cholesteatoma was 65%;and there were almost no positive cells in normal external ear canal skin.p21WAF1 positive cells were detected in each layers,its LI was 28.9%±6.7%.Compared with normal ear canal skin,the expression level of p21WAF1 in middle ear cholesteatoma epithelium was higher than that in normal ear canal skin(P<0.05).③ The TGF-β1 positive rate was 90% in middle cholesteatoma samples and 50% in normal ear canal skin.The positive cells were mostly in spine layer and granular layer in normal ear canal skin,low intensive,light-brown,and its LI was 13.3%±4.9%;in middle ear cholsteatoma epithelium,the positive cells scattered among all layers of the epithelium,and stained strongly in spine layer,high intensive,strong-brown,and its LI was 32.3%±5.7%,there was obvious difference (P<0.05).④In cholesteatoma epithelium samples,the LI of p21WAF1 and TGF-β1 didn't have significant difference.But in cholesteatomatous tissues there was positive correlation between the LI average of p21WAF1 and the LI average of TGF-β1 (r=0.913,P<0.001) .Conclusion TGF-β1 and p21WAF1 can promote the occurrence and development of middle ear cholesteatoma,and they have positive correlation.