白介素17A受体在系统性红斑狼疮患者外周血B淋巴细胞的表达及意义

目的:研究白介素17A受体(IL-17AR)在系统性红斑狼疮(SLE)患者外周血B淋巴细胞内的表达情况及其临床意义。方法:流式细胞术检测60例SLE患者及33例健康人外周血B细胞上IL-17AR的表达水平。并将其与有关临床及实验室指标进行相关性分析。结果:SLE患者组的IL-17AR+细胞表达比例为(47.58±17.20)%,高于正常对照组(40.71±11.82)%(P<0.05)。在SLE患者中:口腔溃疡组、有浆膜炎组、有肾脏病变组、有免疫异常组分别高于相应的无症状组(P<0.05)。在抗Sm-D1抗体阳性组及抗核小体抗体阳性组分别高于相应的抗体阴性组(P<0.05)。IL-17AR+细胞比例与SLE疾病活动指数(SLEDAI)、C反应蛋白(CRP)、甘油三酯呈正相关;与间接胆红素、血清白蛋白的表达负相关(P<0.05)。B淋巴细胞比例与血清IgG、ALT、直接胆红素的表达呈正相关;与胆固醇、甘油三酯、低密度脂蛋白的表达呈负相关(P<0.05)。结论:IL-17AR在SLE患者B细胞上表达上调,且与病情有一定的相关性。提示IL-17/IL-17AR可能在SLE发病中起着重要作用。

The expression and clinical significance of IL-17AR on peripheral blood B cells of patients with systemic lupus erythematosus

AIM: To investgate the expressions of Interleukin-17A receptor(IL-17AR) on the peripheral blood B lymphocytes of SLE patients and to analyze the correlations between IL-17AR and clinical parameters.METHODS: Expression of IL-17AR on peripheral blood CD19 + B lymphocytes were analyzed in 60 SLE patients and 33 healthy controls by flow cytometry.The difference of IL-17AR expression levels in two groups were compared.Furthermore,the correlation between IL-17AR expressions and clinical some measures,such as ESR,CRP,complement 3(C3),complement 4(C4),the amount of serum IgG,anti double strands DNA antibody,anti nuclear antibody,SLEDAI score and urine protein excretion were analyzed.RESULTS: Compared with healthy subjects,the proportions of B cells expressing IL-17AR were higher among SLE patients.In SLE patients,groups with mouth ulcer,serositis,renal lesions or immunologic abnormality were higher than the negative groups separately.The positive correlations were observed between IL-17AR expression levels and clinical measure of the SLEDAI,CRP and serum triglyceride level.The negative correlation was observed between IL-17AR expression levels and clinical measure of the serum indirect bilirubin level,serum albumin level.CONCLUSION: Interleukin-17A receptor expression increased on peripheral blood B cells of SLE patients,and correlate with clinical measures.