子宫内膜腺癌组织中cyclinD1、PCNA和Ki-67的表达

目的　探讨cyclinD1、PCNA和Ki 6 7在子宫内膜腺癌组织中的表达及其意义。 方法　采用免疫组化S P法检测正常增生期子宫内膜 10例、单纯性增生 30例、复杂性增生 30例、非典型增生 30例和子宫内膜腺癌 4 7例组织中cyclinD1、PCNA和Ki 6 7的表达。结果　cyclinD1在增生期子宫内膜组织中未见阳性表达 ,在单纯性增生、复杂性增生、非典型增生和子宫内膜腺癌组织中的阳性表达率分别为 13 3%、16 7%、30 0 %和 5 7 8%。非典型增生和子宫内膜腺癌组织阳性表达率高于增生期宫内膜、单纯性和复杂性增生 (P <0 0 5 ) ,cyclinD1蛋白表达与子宫内膜腺癌临床分期、肌层浸润和淋巴结转移呈正相关 (P<0 0 5 ) ,而与肿瘤的分化程度无关 (P >0 0 5 )。PCNA和Ki 6 7在非典型增生组标记指数 (LI)高于增生期宫内膜、单纯性和复杂性增生组 ,子宫内膜腺癌组高于非典型增生组 (P <0 0 5 ) ,PCNA和Ki 6 7LI与肿瘤的分化程度、临床分期、肌层浸润和淋巴结转移呈正相关 (P <0 0 5 )。PCNA和Ki 6 7在cyclinD1阳性表达组的LI高于阴性组 (P <0 0 5 )。结论　cyclinD1蛋白过度表达可能在子宫内膜癌的发生、发展中起重要作用 ,其作用途径可能是通过促进细胞增殖而实现的。

Expression of cyclinD1, PCNA and Ki-67 in endometrial adenocarcinoma and its significance

Purpose To explore expression of cyclinD1, PCNA and Ki-67 in endometrial adenocarcinoma and its clinical significance. Methods Expression of cyclinD1, PCNA and Ki-67 was detected in 10 cases of normal endometrium, 30 cases of simple hyperplasia, 30 cases of complex hyperplasia, 30 cases of atypical hyperplasia and 47 cases of endometrial adenocarcinoma by immunohistochemical S-P assay. Results There was no positive expression of cyclinD1 in normal endometrium, the positive expression rates of cyclinD1 in simple hyperplasia, complex hyperplasia, atypical hyperplasia and endometrial adenocarcinoma were 13.3%,16.7%,30.0% and 57.8% respectively. There was a significant difference between atypical hyperplasia, endometrial adenocarcinoma and normal endometrium, simple hyperplasia or complex hyperplasia (P<0.05). The expression rate of cyclinD1 had positive correlation with clinical stage, muscles invasion and lymph node metastasis (P<0.05),but not with differentiated degree (P>0.05). The label index of PCNA and Ki-67 in atypical hyperplasia were higher than those in normal endometrium, simple hyperplasia or complex hyperplasia, endometrial adenocarcinoma higher than atypical hyperplasia (P<0.05). The label index of PCNA and Ki-67 was correlated with differentiated degree, histological grade, clinical stage, muscles invasion and lymph node metastasis (P<0.05). The label index of PCNA and Ki-67 with positive cyclinD1 were higher than those with negative cyclinD1 (P<0.05). Conclusion The overexpression of cyclinD1 may play an important role in the carcinogenesis and progression in endometrial carcinoma, and its role may be accomplished by accelerating cellular proliferation.