| 动脉粥样硬化大鼠“痰瘀”病理演变与相关基因表达研究 |
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| 引用本文： | 王东生,袁肇凯,黄献平,卢芳国,陈方平,黄细莲. 动脉粥样硬化大鼠“痰瘀”病理演变与相关基因表达研究[J]. 中华中医药杂志, 2009, 0(5) |
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| 作者姓名： | 王东生 袁肇凯 黄献平 卢芳国 陈方平 黄细莲 |
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| 作者单位： | 中南大学湘雅医院;湖南中医药大学中医诊断学研究所; |
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| 基金项目： | 中国博士后科学基金(No.2003033474,No.20060390891); 湖南省中医药管理局(No.6302-23) |
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| 摘 要： | 目的:探讨动脉粥样硬化(atheroselerosis,AS)大鼠痰瘀病理演变规律和形成机制。方法:复制AS模型大鼠,通过光镜、电镜观察主动脉组织学改变;运用RT-PCR法检测相关基因表达。结果:随着AS的进展,血脂增高、血液流变学异常逐渐加重;在造模第17天脂质代谢明显异常,造模第22天血液流变学显著异常,第32天动脉硬化程度达到2级;基因c-myc、PDGF-A、c-fos、Bcl-2mRNA表达量依次上调,p53mRNA表达下调。结论:基因c-myc、PDGF-A、c-fos、Bcl-2mRNA表达上调、p53 mRNA表达下调可能是AS痰瘀病理演变的分子基础。
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| 关 键 词： | 动脉粥样硬化 动物模型 “痰瘀”病理 血脂 血液流变学 基因表达 |
Study on pathological mechanism of phlegm and blood stasis syndrome and gene expression in atherosclerotic rat |
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| WANG Dong-sheng,YUAN Zhao-kai,HUANG Xian-ping,LU Fang-guo,CHEN Fang-ping,HUANG Xi-lian. Study on pathological mechanism of phlegm and blood stasis syndrome and gene expression in atherosclerotic rat[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2009, 0(5) |
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| Authors: | WANG Dong-sheng YUAN Zhao-kai HUANG Xian-ping LU Fang-guo CHEN Fang-ping HUANG Xi-lian |
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| Affiliation: | WANG Dong-sheng1,YUAN Zhao-kai2,HUANG Xian-ping2,LU Fang-guo2,CHEN Fang-ping1,HUANG Xi-lian1(1 Xiangya Hospital Affi liated to Central South University,Changsha 410008,China,2Institute of Chinese Medical Diagnosis,Hunan University of Traditional Chinese Medicine,Changsha 410007,China) |
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| Abstract: | Objective:To explore the molecular mechanisms of atherosclerosis (AS). Methods: AS rat model was made, arterial morphological histology changes were observed under optic and electronic microscope, and some genes were detected by RT-PCR. Results: There was biochemical change in the blood stasis-phlegm syndrome, which was associated with the alterations of tissue structure. Fat metabolism was obviously abnormal on the 17th day after establishing model in rats.Blood rheology was obviously abnormal on the 22th ... |
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| Keywords: | Atherosclerosis Animal model pathology of phlegm and stasis Blood lipid Hemorheology Gene expression |
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