| 人脐血单个核细胞的体外培养观察 |
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| 引用本文： | 索耀君,王春芳,马韬,秦博,李鑫楠,吴启龙,郭宇宁,李静,李鹏飞,窦岩,赵嘉惠,田树华. 人脐血单个核细胞的体外培养观察[J]. 山西医科大学学报, 2006, 37(9): 923-926 |
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| 作者姓名： | 索耀君 王春芳 马韬 秦博 李鑫楠 吴启龙 郭宇宁 李静 李鹏飞 窦岩 赵嘉惠 田树华 |
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| 作者单位： | 1. 山西医科大学医学实验中心分子生物学实验室,太原,030001
2. 天津医科大学 |
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| 基金项目： | 山西省科技发展基金;山西医科大学校科研和教改项目 |
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| 摘 要： | 目的对脐血间充质干细胞进行体外培养，以进一步阐明其生物学特性，为今后的基础研究和临床应用打好基础。方法采用DMEM培养基培养脐血单个核细胞，流式细胞仪检测细胞表面标志。结果脐血单个核细胞在体外培养24d时，细胞表面CD11b、CD34、CD44和CD45表达阴性，GFAP和nestin表达也为阴性。结论培养所得的单个核细胞区别于脐血中的造血干细胞。
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| 关 键 词： | 胎血 间质细胞 造血干细胞 细胞培养 |
| 文章编号： | 1007-6611（2006）09-0923-04 |
| 收稿时间： | 2006-08-23 |
| 修稿时间： | 2006-08-23 |
Culture of mononuclear cells derived from human umbilical cord blood |
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| SUO Yao-jun, WANG Chun-fang, MA Tao, et al. Culture of mononuclear cells derived from human umbilical cord blood[J]. Journal of Shanxi Medical University, 2006, 37(9): 923-926 |
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| Authors: | SUO Yao-jun WANG Chun-fang MA Tao et al |
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| Abstract: | Objective To study the cultivation and bionomics of mononuclear cells derived from human umbilical cord blood. Methods The mononuclear cells derived from human umbilical cord blood were cultivated and harvested in DMEM, and its signal of cell surface was detected by flow cytometry. Results Following 24 d culture in vitro, the mononuclear cells derived from human umbilical cord blood did not express CD11b, CD34, CD44, CD45, GFAP, and nestin. Conclusion The mononuclear cells derived from human umbilical cord blood are different from hemopoietie stem cells. |
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| Keywords: | fetal blood stromal cells hernatopoietic stem cells cdl culture |
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