骨科病房骨关节感染部位分离病原体及其耐药性

目的了解骨科病房骨关节感染部位分离病原体及其耐药性,为临床预防及治疗骨关节感染提供依据。方法调查某院2010年6月—2013年10月骨科病房送检的114例骨关节感染部位标本,对其病原体及其耐药性进行分析。结果共分离病原体145株,革兰阴性(G-)菌90株(62.07%),革兰阳性(G+)菌50株(34.48%),假丝酵母菌属3株(2.07%),厌氧菌2株(1.38%)。其中居前4位的病原体是铜绿假单胞菌、金黄色葡萄球菌、大肠埃希菌和阴沟肠杆菌,分别占20.69%、12.41%、11.03%、9.65%。主要病原体呈现明显的耐药性,铜绿假单胞菌对头孢他啶耐药率达40.00%;大肠埃希菌对复方磺胺甲口恶唑、环丙沙星和左氧氟沙星耐药,耐药率均达100.00%。结论 G-菌是骨科病房主要病原菌,且分离出的菌株对多种常用抗菌药物耐药。

Distribution and antimicrobial resistance of pathogens isolated from bone and joint infection sites of orthopedic patients

ObjectiveTo understand the distribution and antimicrobial resistance of pathogens isolated from bone and joint infection sites  of  orthopedic patients, so as to provide reference for  prevention and therapy of bone and joint infection. Methods114 specimens from bone and joint infection sites of orthopedic patients between June 2010 and October 2013 were surveyed, pathogens and antimicrobial susceptibility of pathogens were analyzed.ResultsA total of 145 pathogenic isolates were obtained, 62.07%(n=90) of which were gram negative bacteria,34.48%(n=50) were gram positive bacteria, 2.07%(n=3) were Candida spp., and 1.38%(n=2) were anaerobic bacteria. The top 4 pathogens were Pseudomonas aeruginosa (20.69%), Staphylococcus aureus (12.41%), Escherichia coli (11.03 %), and Enterobacter cloacae (9.65%).These pathogens presented remarkable resistance. The resistant rate of Pseudomonas aeruginosa to ceftazidime was up to 40.00%; the resistant rates of Escherichia coli to compound sulfamethoxazole, ciprofloxacin and levofloxacin were all 100.00%.ConclusionGram negative bacteria are the major pathogens in orthopedic ward, the isolated strains are resistant to multiple antimicrobial agents.