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| 组蛋白去乙酰化酶在食管鳞癌中的表达及其表达下调对EC9706细胞生物特性的影响 | |
| 引用本文： | 张娜,陈晓琦,刘红涛,席宇,李晟磊.组蛋白去乙酰化酶在食管鳞癌中的表达及其表达下调对EC9706细胞生物特性的影响[J].肿瘤,2011,31(10):886-892. |
| 作者姓名： | 张娜 陈晓琦 刘红涛 席宇 李晟磊 |
| 作者单位： | 1. 郑州大学第一附属医院病理科,郑州450052;郑州大学基础医学院病理教研室,郑州 450001 2. 商丘医学高等专科学校病理教研室,商丘,476100 3. 郑州大学生物工程系,郑州,450001 |
| 摘 要： | 目的:探讨组蛋白去乙酰化酶(histone deacetylase2,HDAC2)在食管鳞癌组织中的表达,并研究其表达下调对食管鳞癌EC9706细胞增殖、细胞周期和细胞凋亡的影响,以及分析其相关的分子机制。方法:采用免疫组织化学法检测食管鳞癌组织中HDAC2蛋白的表达。将特异性针对HDAC2基因的小分子干扰RNA(small interfering RNA,siRNA)和对照siRNA分别转染食管鳞癌EC9706细胞,实验分3组:未处理组、对照siRNA组和HDAC2siRNA组。蛋白质印迹法检测各组EC9706细胞中HDAC2蛋白的表达。CCK-8计数法检测转染前后细胞的增殖情况。FCM法检测细胞周期和细胞凋亡的变化。蛋白质印迹法检测与细胞增殖、细胞周期和细胞凋亡相关蛋白的表达变化。结果:HDAC2蛋白在食管鳞癌组织中表达的阳性率为79.71%,显著高于癌旁不典型增生组织的51.11%和正常食管黏膜组织的23.19%,3者之间差异具有统计学意义(χ2=44.121,P=0.000);此外,HDAC2蛋白表达与患者的年龄和性别无关(P均>0.05),但是与组织学分级、浸润深度、TNM分期和淋巴结转移均显著相关(P均<0.05)。HDAC2siRNA能有效下调食管鳞癌EC9706细胞中HDAC2蛋白的表达,明显抑制食管鳞癌EC9706细胞的增殖,促使细胞周期静止在G0/G1期,并诱导细胞凋亡。蛋白质印迹法检测结果显示,HDAC2表达下调能明显提高p21和凋亡相关蛋白bax的表达量,同时降低细胞周期蛋白cyclin D1和bcl-2的表达量。结论:HDAC2可能在食管鳞癌的发生、发展中具有重要作用,其表达下调介导的食管鳞癌细胞增殖抑制、细胞周期静止以及细胞凋亡可能与p21、bax的表达升高和cyclin D1、bcl-2表达降低密切相关。 |
| 关 键 词： | 食管肿瘤 组蛋白脱乙酰基酶类 RNA 小干扰 细胞增殖 细胞凋亡 |
The downregulation of HDAC2 expression in esophageal squamous cell carcinoma and its effect on biological features of EC9706 cells |
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| ZHANG Na,CHEN Xiao-qi,LIU Hong-tao,XI Yu,LI Sheng-lei.The downregulation of HDAC2 expression in esophageal squamous cell carcinoma and its effect on biological features of EC9706 cells[J].Tumor,2011,31(10):886-892. | |
| Authors: | ZHANG Na CHEN Xiao-qi LIU Hong-tao XI Yu LI Sheng-lei |
| Institution: | ZHANG Na1,2,CHEN Xiao-qi3,LIU Hong-tao4,XI Yu4,LI Sheng-lei1,2 1.Department of Pathology,First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China,2.Department of Pathology,Basic Medical College of Zhengzhou University,Zhengzhou 450001,3.Department of Pathology,Medical College of Shangqiu,Shangqiu 476100,4.Department of Bioengineering,Zhengzhou University |
| Abstract: | Objective:To investigate the expression of histone deacetylase 2(HDAC2) in esophageal squamous cell carcinoma(ESCC),and to explore the effects of downregulation of HDAC2 expression on cell proliferation,cell cycle and apoptosis of EC9706 cells and their possible molecular mechanisms.Methods:The expression of HDAC2 protein in ESCC tissues was detected by immunohistochemistry.HDAC2 small interfering RNA(siRNA) and control siRNA were transfected into ESCC EC9706 cells,and then the EC9706 cells were randomly di... |
| Keywords: | Esophageal neoplasms Histone deacetylases RNA small interfering Cell proliferation Apoptosis |
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