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| 南蛇藤总萜抑制荷肝癌HepA1-6小鼠移植瘤的生长 | |
| 引用本文： | 侯莹,员林,钱亚云,张华,刘延庆. 南蛇藤总萜抑制荷肝癌HepA1-6小鼠移植瘤的生长[J]. 肿瘤, 2011, 31(11): 999-1003. DOI: 10.3781/j.issn.1000-7431.2011.11.007 |
| 作者姓名： | 侯莹 员林 钱亚云 张华 刘延庆 |
| 作者单位： | 1. 溧水县人民医院肿瘤科,南京211200;扬州大学中西医结合研究所,扬州225009 2. 扬州大学中西医结合研究所,扬州225009;中国人民解放军第97医院影像科,徐州221000 3. 扬州大学中西医结合研究所,扬州,225009 4. 扬州大学医学院护理系,扬州,225009 |
| 摘 要： | 目的:探讨南蛇藤总萜对荷肝癌HepA1-6小鼠移植瘤生长的影响及其可能机制。方法:建立肝癌HepA1-6小鼠移植瘤模型,分为空白对照组、溶媒对照组(1%DMSO)、阴性对照组(0.9%氯化钠溶液)、阳性对照组(顺铂)及南蛇藤总萜低(10 mg/kg)、中(20 mg/kg)和高(40 mg/kg)剂量组。观察南蛇藤总萜对小鼠体质量、抑瘤率、肝脏指数、脾脏指数及胸腺指数的影响;应用免疫组织化学法检测移植瘤组织中血管内皮生长因子(vascular end othelial growth factor,VEGF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的表达以及微血管密度(microvessel density,MVD)计数;TUNEL法检测移植瘤组织中的细胞凋亡。结果:南蛇藤总萜可明显抑制荷肝癌HepA1-6小鼠移植瘤的生长,促进肿瘤细胞凋亡,下调移植瘤组织中VEGF和bFGF的表达以及MVD计数,与阴性对照组比较,差异有统计学意义(P<0.05);南蛇藤总萜组小鼠肝、脾和胸腺指数及体质量与阴性对照组比较,差异无统计学意义(P>0.05)。结论:南蛇藤总萜可明显抑制荷肝癌HepA1-6小鼠移植瘤的生长,可能与其促进肿瘤细胞凋亡及抑制肿瘤血管生成有关。 |
| 关 键 词： | 肝肿瘤 模型,动物 新生血管化,病理性 南蛇藤 小鼠 |
Celastrus orbiculatus extract inhibits the xenograft tumor growth of HepA1-6 hepatoma in mice |
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| HOU Ying,YUAN Lin,QIAN Ya-yun,ZHANG Hua,LIU Yan-qing. Celastrus orbiculatus extract inhibits the xenograft tumor growth of HepA1-6 hepatoma in mice[J]. Tumor, 2011, 31(11): 999-1003. DOI: 10.3781/j.issn.1000-7431.2011.11.007 | |
| Authors: | HOU Ying YUAN Lin QIAN Ya-yun ZHANG Hua LIU Yan-qing |
| Affiliation: | HOU Ying1,2,YUAN Lin2,3,QIAN Ya-yun2,ZHANG Hua4,LIU Yan-qing2 1.Department of Oncology,Lishui Country People's Hospital,Nanjing 211200,China,2.Institute of Traditional Chinese Medicine & Western Medicine,Yangzhou University,Yangzhou 225009,3.Department of Radiology,97th Hospital of People's Liberation Army,Xuzhou 221000,4.Department of Nursing,Medical College |
| Abstract: | Objective:To investigate the effect of Celastrus orbiculatus extracts(COE) on xenograft tumor growth of HepA1-6 hepatoma in mice,and to explore its possible mechanism.Methods:HepA1-6 hepatoma model was established in mice,and then these mice were randomly divided into blank control group,solvent control group(1% DMSO),negative control group(0.9% NaCl solution),positive control group(cisplatin),and low-,medium-and high-dose COE groups(10,20 and 40 mg/kg,respectively).The body weight,tumor growth inhibitory r... |
| Keywords: | Liver neoplasms Models animal Neovascularization pathologic Celastrus orbiculatus Mice |
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