不同侵袭转移潜能浆液性卵巢癌细胞株3AO生物活性及蛋白质质谱研究

 目的：筛选与卵巢癌侵袭转移特性相关的蛋白质。方法：将人浆液性卵巢癌细胞系3AO在裸鼠体内多次传代，体外培养建系，结合细胞粘附、侵袭及移动实验筛选高、低侵袭潜能细胞亚系。采用表面增强激光解吸电离-飞行时间-质谱（SELDI-TOF-MS）检测二者的细胞裂解液，比较其蛋白质指纹图谱。结果：通过体内、外实验筛选出细胞亚系3AOF3、3AOF4，与其母系3AO相比具有高侵袭潜能。比较三者蛋白质指纹图谱发现: 发现9个差异蛋白质峰(M/Z分别为3494、3831、8121、8293、9123Da、9332、10184、15436和17134)仅在母系3AO细胞株中表达。相反，有3个差异蛋白质峰(M/Z分别为1842、3145和4360）仅在F3、F4代中表达。6个差异蛋白质峰（1236、5476、5649、6575、6897和7016）和1组蛋白质谱峰簇在母系3AO细胞株和3AOF1－4细胞中均可表达，但强度不同。结论：通过比较同一遗传背景的高、低侵袭潜能卵巢癌细胞的蛋白质指纹图谱，发现一组与侵袭潜能密切相关的差异蛋白质峰。

Analysis of proteomic fingerprints of the ovarian cancer 3AO cell line with different invasive potential

Objective To screen for proteins related to invasive and metastatic potential of ovarian cancer.Methods The nude mice were intraperitoneally injected with the human ovarian cancer 3AO cell line.The metastatic tumors were isolated and cultured in vitro.The cell lines were established from tumors and transplanted intraperitoneally for several passages.Invasive potential of cell lines was evaluated by cell adhesion and matrigel invasion assays.Lysates from different cell lines with high or low invasive potential were analyzed by surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS)and protein-chip array technology. The proteomic fingerprint patterns were compared. Results The subclones 3AOF3 and 3AOF4 were isolated from repeated passaging in vivo and vitro,and showed higher invasive capacity than 3AO cells.By comparing their proteomic fingerprints,9 distinct proteins (3494,3831,8121,8293,9123,9332,10 184,15 436 and 17 134 at m/z value) were found,which were expressed only in 3AO cells.On the contrary,1 distinct protein (4360 at m/z value) was expressed only in 3AO F3-4 cells,and 2 distinct proteins (1842 and 3145 at m/z value) were expressed only in 3AO F2-4 cells.Additionally,6 distinct proteins (1236,5476,5649,6575,6897 and 7016 at m/z value) and a mass cluster (13 794 and 14 027 at m/z value) were expressed in all 3AO and 3AOF1-4 cells,while the amount of protein was different.Conclusion By comparing the proteomic fingerprints of isogenic ovarian cancer cells with different invasive potential,a group of proteins are identified which are correlated with high invasive and metastatic potential.