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| VEGF和Smac/DIABLO对胶质瘤细胞凋亡及化疗敏感性的影响 | |
| 引用本文： | 陈音,马杰,吴国华,蔡威.VEGF和Smac/DIABLO对胶质瘤细胞凋亡及化疗敏感性的影响[J].肿瘤,2011,0(9):800-805. |
| 作者姓名： | 陈音 马杰 吴国华 蔡威 |
| 作者单位： | 1. 温州医学院,温州,325035 2. 上海交通大学医学院附属新华医院儿外科,上海,200092 3. 上海交通大学医学院附属新华医院肿瘤科,上海,200092 |
| 摘 要： | 目的:旨在研究血管内皮生长因子(vascular endothelial growth factor,VEGF)和第二线粒体衍生的caspase激活剂(second mitochondrial activator of caspase,Smac)/低等电点凋亡抑制蛋白直接结合蛋白(direct IAP binding protein with low PI,DIABLO)在胶质瘤发生和发展中的作用。方法:采用慢病毒介导的RNA干扰技术沉默VEGF基因在胶质瘤细胞U251中的表达;分别采用实时荧光定量PCR(real-time?uorogenic quantitative-PCR,RFQ-PCR)和蛋白质印迹法检测VEGF和Smac/DIABLO mRNA及蛋白在U251细胞中的表达水平;采用蛋白质印迹法检测磷酸化细胞外蛋白调节激酶(extracellular regulated protein kinase,ERK)的表达;FCM检测VEGF沉默对细胞周期的影响;MTT法检测VEGF沉默对U251细胞化疗敏感性的影响。结果:VEGF基因沉默可导致VEGF和Smac/DIABLO mRNA及蛋白表达水平的下调,并抑制磷酸化ERK的表达,提示VEGF是Smac/DIABLO的上游调节因子,通过激活ERK的信号通路调节Smac/DIABLO的表达水平。VEGF基因沉默导致U251细胞中处于S期的细胞比例增多,并增强顺铂对U251细胞的凋亡诱导作用。结论:VEGF可影响胶质瘤细胞周期的分布以及对顺铂的化疗敏感性。Smac/DIABLO参与了VEGF信号通路。 |
| 关 键 词： | 神经胶质瘤 RNA干扰 血管内皮生长因子 第二线粒体衍生的caspase激活剂/低等电点凋亡抑制蛋白直接结合蛋白 细胞 U251 |
The effects of VEGF and Smac/DIABLO on apoptosis and chemosensitivity of human glioma cells |
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| CHEN Yin,MA Jie,WU Guo-hua,CAI Wei.The effects of VEGF and Smac/DIABLO on apoptosis and chemosensitivity of human glioma cells[J].Tumor,2011,0(9):800-805. | |
| Authors: | CHEN Yin MA Jie WU Guo-hua CAI Wei |
| Institution: | CHEN Yin1,MA Jie2,WU Guo-hua3,CAI Wei2 1.Wenzhou Medical College,Wenzhou 325035,China,2.Department of Pediatric Surgery,Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 200092,3.Department of Oncology |
| Abstract: | Objective:To investigate the roles of vascular endothelial growth factor(VEGF) and second mitochondrial activator of caspase(Smac) /direct IAP binding protein with low PI(DIABLO) in occurrence and development of glioma.Methods:The expression of VEGF gene was down-regulated in glioma U251 cells by lentivirus-mediated RNA interference.The expressions of VEGF and Smac/DIABLO mRNAs and protein were analyzed by real-time fluorogenic quantitative-PCR and Western blotting,respectively.The expression of phosphoryla... |
| Keywords: | Glioma RNA interference Vascular endothelial growth factor Second mitochondrial activator of caspase(Smac) /direct IAP binding protein with low PI Cell U251 |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |