蟾蜍毒素联合紫杉醇抑制人胃癌MGC803细胞增殖和诱导凋亡的作用

目的探讨蟾蜍毒素联合紫杉醇抑制人胃癌MGC803细胞增殖及诱导凋亡的作用。方法应用MTT法检测不同浓度蟾蜍毒素及紫杉醇单药及联合对胃癌细胞MGC803增殖抑制作用；流式细胞术分析细胞凋亡和周期阻滞；Westernblot法检测C-FLIPL、Caspase-8蛋白的表达。结果(1)蟾蜍毒素及紫杉醇单药均抑制MGC803细胞增殖，呈剂量-时间依赖效应，且联合用药的细胞增殖抑制率明显高于单药组，差异有统计学意义(P<0.05)。24、48及72 h联合指数(CI)分别为0.77、0.86、0.72；(2) 40 nmol/L蟾蜍毒素、25 ng/ml紫杉醇单药及联合作用细胞24 h，流式细胞仪检测凋亡率分别为14.13%、19.74%及53.30%，联合组与单药组比较差异有统计学意义(P<0.05)；(3)Western blot结果显示，蟾蜍毒素、紫杉醇单药组及联合组作用细胞24 h后，C-FLIPL蛋白表达依次下降至对照组的78.38%、64.71%、46.73%，Caspase-8蛋白表达上调至对照组的150.25%，156.86%，180.58%。结论蟾蜍毒素协同紫杉醇诱导胃癌MGC803细胞凋亡，且可能与下调C-FLIP、上调Caspase-8蛋白有关。

Bufalin and Paclitaxel Inhibited Proliferation and Induced Apoptosis of Gastric Cancer Cell Line MGC803

Objective To investigate the effects of bufalin and Paclitaxel on proliferation and apoptosis of gastric cancer cell line MGC803.Methods MGC803 treated by various concentrations of bufalin and Paclitaxel alone or in combination.The inhibition rate of cell growth detected by MTT assay,the apoptosis by flow cytometry and the protein expression of cell apoptosis associated proteins C-FLIPL and Caspase-8 by Western blot.Results Bufalin or Paclitaxel effectively inhibited the growth of MGC803 gastric cancer cell...