| 靶向EGFR基因的shRNA抑制胰腺癌PANC-1细胞增殖的研究 |
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| 引用本文： | 林远洪,雷小林,吴永忠,高泽莉.靶向EGFR基因的shRNA抑制胰腺癌PANC-1细胞增殖的研究[J].肿瘤防治研究,2011,38(9):1012-1015. |
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| 作者姓名： | 林远洪 雷小林 吴永忠 高泽莉 |
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| 作者单位： | 1.617000 四川攀枝花，攀枝花学院附属医院肿瘤科；2.重庆市肿瘤研究所 |
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| 摘 要： | 目的探讨EGFR基因对胰腺癌PANC-1细胞的增殖抑制作用。方法构建针对EGFR序列特异性shRNA的表达载体，用脂质体转染胰腺癌PANC-1细胞。采用RT-PCR、Western blot检测EGFR mRNA和蛋白的表达；流式细胞仪检测细胞周期及凋亡；克隆形成实验检测细胞增殖。结果靶向EGFR的序列特异性shRNA明显抑制EGFR mRNA和蛋白的表达，EGFR mRNA和蛋白的抑制率分别为72.1％和67.6％；G1期细胞增多、S期细胞减少(P<0.05)；细胞凋亡增加(P<0.05)；克隆形成减少(P<0.05)。结论靶向EGFR的序列特异性shRNA能明显抑制胰腺癌细胞增殖、促进凋亡。
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| 关 键 词： | RNAi 表皮生长因子受体 胰腺癌 |
| 收稿时间： | 2010-08-23; |
Inhibition Effect of Pancreatic Cancer PANC-1 Cells by shRNA Targeting on EGFR |
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| LIN Yuan-hong,LEI Xiao-lin,WU Yong-zhong,GAO Ze-li.Inhibition Effect of Pancreatic Cancer PANC-1 Cells by shRNA Targeting on EGFR[J].Cancer Research on Prevention and Treatment,2011,38(9):1012-1015. |
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| Authors: | LIN Yuan-hong LEI Xiao-lin WU Yong-zhong GAO Ze-li |
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| Institution: | 1.Department of Tumor,The Affiliated Hospital of Panzhihua University,Panzhihua 617000,China;2.Chongqing Tumor Institute |
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| Abstract: | Objective To explore the effect of EGFR gene on proliferation pancreatic cancer PANC-1 cells.Methods EGFR gene sequence-specific shRNA expression vector was constructed and then transfected into pancreatic cancer PANC-1 cells with lipofectamine.The EGFR mRNA and protein expression was detected by RT-PCR and Western blot.The cell cycle distribution and apoptosis were detected by cell flow cytometry.The proliferation of cells was detected by clone formation assay.Results Sequence-specific shRNA targeting on E... |
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| Keywords: | RNA interference EGFR Pancreatic cancer |
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