| 人骨髓基质干细胞体外诱导为成骨细胞的实验研究 |
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| 引用本文： | 吴云刚,张志平.人骨髓基质干细胞体外诱导为成骨细胞的实验研究[J].江西中医学院学报,2007,19(1):74-75. |
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| 作者姓名： | 吴云刚 张志平 |
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| 作者单位： | 1. 浙江省温州医学院一附院骨伤科,温州,325000
2. 江西省南昌市第一医院骨科,南昌,330008 |
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| 摘 要： | 目的:探讨体外培养的人骨髓基质干细胞(hBMSCs)诱导分化为成骨细胞的方法。方法:抽取骨髓,用梯度离心法分离单个核细胞,用含10%胎牛血清的DMEM普通培养基培养,传代后改用含β-甘油磷酸钠、维生素C和地塞米松的条件培养基培养,用相差显微镜下观察细胞形态,钙钴法碱性磷酸酶染色,Von Kossa法钙结节染色及细胞内ALP含量测定。结果:形态学观察、碱性磷酸酶染色、钙结节染色及细胞内ALP含量测定均表明,hBMSCs经条件培养基培养后诱导的细胞具有成骨细胞的形态学及生物学特性。结论:hBMSCs体外经含β-甘油磷酸钠、维生素C和地塞米松的条件培养基培养可诱导为成骨细胞。
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| 关 键 词： | 骨髓基质干细胞 成骨细胞 碱性磷酸酶 |
| 收稿时间： | 2006-07-29 |
| 修稿时间： | 2006年7月29日 |
Experiments on hBMSCs induced to differentiate into osteoblasts in vitro |
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| WU Yun-gang,ZHANG Zhi-ping.Experiments on hBMSCs induced to differentiate into osteoblasts in vitro[J].Journal of Jiangxi College of Traditional Chinese Medicine,2007,19(1):74-75. |
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| Authors: | WU Yun-gang ZHANG Zhi-ping |
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| Institution: | 1. The First Affiliated Hospital of Wenzhou University, Wenzhou 325000; 2. Nanchang First Hospital ,Jiangxi Prounnce , Nanchang 330006 |
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| Abstract: | Objective:To investigate hBMSCs can be induced to differentiate into osteoblasts in vitro.Methods:The hBMSCs were separated by gradient centrifugation on Percoll,cultured with ordinary culture medium and induced by conditional culture medium.The cells cultured with ordinary and conditional culture medium were examined by microscopy,ALP staining,Von-Kossa staining and ALP assay.Results:Microscopy,ALP staining,Von-Kossa staining and ALP assay all show that the cells cultured with conditional culture medium have the characteristics and the activity of the osteoblasts.Conclusion:hBMSCs can be induced to differentiate into osteoblasts in conditional culture medium in vitro. |
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| Keywords: | Bone marrow stromal cell Osteoblast Alkaline phosphatese |
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