原发性肝癌肿瘤血管密度及其表达的临床病理意义

目的研究肿瘤微血管密度（ＭＶＤ）和肿瘤血管生长因子（ＶＥＧＦ）的表达水平在原发性肝癌中的临床病理意义。方法对６３例手术切除的小肝癌（直径＜５ｃｍ）患者的临床病理资料进行了回顾性的研究。其中２年内转移复发组２９例,２年内无转移复发组３４例。每例取３张连续的切片分别进行ＨＥ染色、ＶＥＧＦ和生物素标记的荆豆凝集素Ｉ（Ｂｉｏ－ＵＥＡ－Ｉ）的免疫组化染色。根据Ｂｉｏ－ＵＥＡ－Ｉ染色的血管内皮细胞计数来测定ＭＶＤ。结果转移复发组和无转移复发组的ＭＶＤ分别为４９．６±２９．７、２２．７±２８．２（Ｐ＜０．０１）;转移复发组和无转移复发组的ＶＥＧＦ阳性率分别为８６．２％（２５／２９）、４７．１％（１６／３４）,两者差异有显著性（Ｐ＜０．０１）。两组在肿瘤分期、有无卫星灶、有无门脉癌栓３方面差异也具有显著性（Ｐ＜０．０５,Ｐ＜０．０５,Ｐ＜０．０１）。结论除肿瘤分期、卫星灶、门脉癌栓具有预后意义外,肝癌组织中ＭＶＤ和ＶＥＧＦ的表达也具有预后价值

Clinico-pathological significance of microvessel density and VEGF expression in primary liver cancer

OBJECTIVE: To evulate the clinico-pathological singnificance of intratumoral microvessel density (MVD) and VEGF expression in primary liver cancer (PLC). METHODS: A retrospective study including 63 postoperative small PLC (diameter < 5 cm) patients was done. One group of 29 patients developed recurrence or metastasis within 2 years. The other group of 34 patients had no evidence of recurrence or metastasis within 2 years. Three PLC sections were taken from each patient, one for H. E. staining, the other two for VEGF and vascular endothelial cell immunohistochemical staining, respectively. MVD was counted by endothelial cells which were highlighted by Bio-UEA-I. RESULTS: The MVD in patients with cancer recurrence or metastasis was (49.6 +/- 29.7) significantly greater than the other group (22.7 +/- 28.2) (P < 0.01). The positive rate of VEGF in cancer recurrence group was 86.2% (25/29), being significantly higher than the other group (47.1%) (P < 0.01). The stage of the tumor, the positive rate of satellite nodules and portal vein embolus were all significantly different between the 2 groups. CONCLUSION: Besides tumor stage, satellite nodules and portal vein embolus, the MVD and VEGF expression are also of prognostic significance.