| 中药红花浸液对植入期子宫内膜白血病抑制因子表达水平的影响 |
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| 引用本文： | 廉瑞青,马保华,王哲民. 中药红花浸液对植入期子宫内膜白血病抑制因子表达水平的影响[J]. 现代妇产科进展, 2007, 16(3): 194-196,I0001 |
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| 作者姓名： | 廉瑞青 马保华 王哲民 |
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| 作者单位： | 1. 山东大学医学院组织学与胚胎学教研室,济南,250012
2. 山东省中医药研究所生物化学室 |
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| 摘 要： | 目的:探讨中药红花抗早孕过程中的生物学作用机理。方法:随机将受精后第2天的孕鼠分为3个实验组和对照组,分别经口给予不同浓度的红花液和生理盐水,连续3天,停药后24天取子宫,石蜡包埋切片,进行HE和免疫组化染色,将染色结果输入图像分析仪,观察子宫内膜的形态和白血病抑制因子(leukemia inhibitory factor,LIF)蛋白的表达,并作定量分析。结果:实验组子宫内膜形态有异常改变;免疫组化染色表明,随着药物剂量的增多,子宫内膜细胞LIF蛋白的表达逐渐减少;但对照组呈强阳性表达。结论:中药红花作用于孕鼠子宫内膜细胞,抑制LIF蛋白的表达,从而影响胚胎着床,起到了抗早孕的作用。
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| 关 键 词： | 红花 白血病抑制因子 子宫内膜 抗早孕 |
| 文章编号： | 1004-7379（2007）03-0194-03 |
| 收稿时间： | 2006-10-24 |
| 修稿时间： | 2006-10-24 |
The effect of Chinese-drug-saffron steep on the expression of leukemia inhibitory factor in the endometrium of implantation |
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| Lian Ruiqing,Ma Baohua,Wang Zhemin. The effect of Chinese-drug-saffron steep on the expression of leukemia inhibitory factor in the endometrium of implantation[J]. Current Advances In Obstetrics and Gynecology, 2007, 16(3): 194-196,I0001 |
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| Authors: | Lian Ruiqing Ma Baohua Wang Zhemin |
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| Abstract: | Objective:To study the biological mechanism of Chinese-drug-saffron during anti-early pregnancy.Method:The mated female mice were divided into three experimental groups,saffon with different concentrations were used on the 2nd day after fertilization,which lasting for 3 days,and the change of the morphology endometrium was observed.The sections were stained with HE and LIF immunohistochemistry,quantitative analysis was proformed.Result:In experimental groups,the endometrium morphology changed significantly and the expression of LIF-protein decreased gradually with the increasing of concentration,whereas the control group stable positive LIF-protein expression.Conclusion:The Chinese-drug-saffron can inhibit the expression of LIF-protein on the endometium and may have a role of anti-early-pregnancy. |
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| Keywords: | Saffron Leukemia inhibitory factor Endometrium Antiearly pregnancy |
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