戊四氮诱导单次惊厥幼鼠海马碱性成纤维细胞生长因子表达的研究

目的利用戊四氮单次惊厥幼鼠模型,研究癫痫单次惊厥发作后海马各区碱性成纤维细胞生长因子（bFGF）表达情况。方法建立戊四氮腹腔注射幼鼠单次惊厥发作模型,分成A组（癫痫发作组）和B组（生理盐水对照组）。每个组后按造模后1天、7天、14天分为3组。HE染色观察病理形态改变,免疫组化观察海马bFGF表达变化。结果病理形态上A组可见海马神经元变性、坏死,胶质细胞增生,B组形态学无明显变化。bFGF表达：A组发作后1天bFGF表达增高最明显（P〈0.01）,以CA1区和齿状回明显,以后逐渐下降,7天时仍高于B组（P〈0.05）,14天时下降到较低水平,和B组无显著性差异（P〉0.05）。B组各时间点bFGF表达无显著性差异。结论幼鼠单次惊厥发作后海马bFGF表达增加,在发作后7天内较为明显。bFGF在癫痫后脑损伤的自我修复中可能发挥着重要的作用。

Expression of basic fibroblast growth factor in the brain of epileptic rat induced with pentetrazole

Objective To study the expression of basic fibroblast growth factor(bFGF) in the hippocampus of epileptic pedo-rats at different time points and investigate its bearing on epilepsy.Methods Forty-two 14-day old SD pedo-rats were randomly divided into two groups: A(epilepsy model group) and B(normal saline group).Pentetrazole was used to induce seizure models.The expression of bFGF in the hippocampus of rats was studied by immunofluorescent labeling technique.H-E staining was done for pathomorphological study.Results In group A,neuronal degeneration and necrosis in the hippocampus,and cellular proliferation of glial cells as well,were noticed;the expression of bFGF was increased,most marked 1 day after seizure(P<0.01),continued to be higher for 7 days(P<0.05),and then declined and approached the control level 14 days later(P>0.05).Conclusion The expression of bFGF in hippocampus of 14-day rat is increased after acute epilepsy,significantly higher for 7 days after seizure.bFGF may play an important role in the self-plerosis after brain injury induced by epilepsy in pedo-rats.