| 幽门螺杆菌flaA基因的变异 |
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| 引用本文： | 余毅,徐智民,欧锡祺,宋姗,张妙英.幽门螺杆菌flaA基因的变异[J].世界华人消化杂志,1999,0(4):323-325. |
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| 作者姓名： | 余毅 徐智民 欧锡祺 宋姗 张妙英 |
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| 作者单位： | 510515,广州市同和南方医院全军消化内科研究所. ilxunuzm@usa.net |
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| 摘 要： | 目的鞭毛是幽门螺杆菌（Ｈｐ）的重要侵袭因子，本研究拟检测Ｈｐ鞭毛素Ａ基因的变异性，进而研究其与Ｈｐ致病性的关系．方法我们对５２例胃粘膜活检标本和１８株Ｈｐ临床分离株进行Ｈｐ特异的１６ＳｒＲＮＡ基因和鞭毛素Ａ基因ＰＣＲ扩增和ＰＣＲＲＦＬＰ及ＰＣＲＲＦＬＰＳＳＣＰ分析．结果对４３例Ｈｐ（＋）的ｆｌａＡ基因ＰＣＲＲＦＬＰ（ＨｉｎｄⅢ）大约可分７个型（变异检出率为１６３％），而ＰＣＲＲＦＬＰＳＳＣＰ可分３７个型（变异检出率为８６０％），两者有非常显著性差异．结论ｆｌａＡ基因变异性极大，ＰＣＲＲＦＬＰＳＳＣＰ优于ＰＣＲＲＦＬＰ，是检测其变异的有效方法．
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| 关 键 词： | 螺杆菌.幽门 鞭毛素A基因 胃疾病 |
| 修稿时间： | 1998-07-07 |
Variability of fla A gene of Helicobacter pylori |
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| YU Yi ,XU Zhi Min ,OU Xi Qi ,SONG Shan and ZHANG Miao Ying.Variability of fla A gene of Helicobacter pylori[J].World Chinese Journal of Digestology,1999,0(4):323-325. |
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| Authors: | YU Yi XU Zhi Min OU Xi Qi SONG Shan and ZHANG Miao Ying |
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| Institution: | YU Yi 1,XU Zhi Min 2,OU Xi Qi 1,SONG Shan 2 and ZHANG Miao Ying 1 1Department of Daliang Hospital,Shunde 510300,Guangdong Province,China 2PLA Institute for Digestive Diseases,Nanfang Hospital,the
First Military Medical |
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| Abstract: | AIM To study the variability and pathogenjcity of Helicobacter pylori (Hp) . METHODS PCR
was performed in fifty two biopsy specimens of gastric mucosa and 18 strains of Hp for 16S
rRNA gene and flagellin A gene ( fla A). And PCR-RFLP and RFLP SSCP were also analyzed.
RESULTS Seven fla A genotypes were found by PCR RFLP in 43 strains of Hp (detecting
rate of variance was 16 3%), but 37 were found by PCR RFLP SSCP in the same group of
cases (detecting rate of variance was 86 0%). CONCLUSION Very large variability in fla A
gene of Hp was detected by PCR RFLP SSCP, which is a more effective method than PCR
RFLP to identify Hp strains in human stomach. |
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| Keywords: | Helicobacter pylori fla A gene stomach diseases |
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