金地鼠颊囊致癌模型P53及其对细胞增殖作用的观察

有效地早期诊断是阻断癌前病变,预防癌变发生的必要条件,本实验建立ＤＭＢＡ（二甲基苯并蒽）诱导的金地鼠颊囊致癌模型,采用Ｐ５３增殖细胞核抗原（ＰＣＮＡ）等免疫组化方法及ＶＩＤＡＳ图像分析,发现Ｐ５３表达能敏感,准确地反映口腔癌前病变的粘膜病变程度,并观察了Ｐ５３阳性细胞在不同程度年皮异常增生间具有不同的分布形态,实验还发现Ｐ５３与ＰＣＮＡ从不同检测角度揭示了细胞动力学变化的同一本质,初步证实了“Ｐ５

Observations of P_(53) and the role of P_(53) in the cell proliferation on golden hamster carcinogenesis model

The effective early diagnosis is essential to obstructing oral precancerous lesions and preventing carcinomatous conversion. An immunohistochemistry with anti-P53 antibody and anti-PCNA antibody. VIDAS image analysis system were applied and observed on golden hamster cheek pouch car-cinogenesis model. The results showed that expression of P53 protein could reflect the severity degrees of the lesions sensitively and accurately, moreover, the distribution of P53-positive cells varied with the grade of epithelial dysplasia: P53 and PCNA disclosed the same essence of cellular dynamics from different points of view, and verified the hypothesis preliminarily, "Once being in loss of function. P53 can promote abnormal cell growth. "; the results also indicated that it was important to control the time of fixation and select the correct way of observation and counting.