无花果残渣中抗肿瘤成分的超临界CO2萃取和抑瘤试验

目的:研究无花果残渣中抗肿瘤功效成分的超临界CO₂萃取技术及萃取物的体内外抗肿瘤效果。方法:采用MTT法检测萃取物质的体外抗肿瘤活性,研究残渣中抗肿瘤功效成分的最佳萃取条件;采用MTT法研究萃取物质对U937细胞、95D细胞和AGS细胞3株肿瘤细胞株的体外抑制作用;以小鼠移植性肝癌Heps为对象,研究萃取物对体内肿瘤的抑制作用。结果:超临界CO₂提取的最佳工艺条件为:粉碎度100目,萃取压强30MPa,萃取温度45℃,萃取时间6h,气体流量12L.h^-1,萃取得率为25.3g.kg^-1,得到的萃取物在体外对U937细胞、95D细胞和AGS细胞具有一定的抑制作用,IC₅₀分别为70.125,127.957,116.000μg.mL^-1。萃取物对小鼠移植性肝癌Heps在25mg.kg^-1的剂量时抑瘤率为49.3%。结论:本萃取工艺合理、稳定。可以为无花果残渣和无花果中抗肿瘤功效成分的提取提供参考。

Study on anti-cancer components of Fig Residues with suppercritical fluid CO2 extracting technique

OBJECTIVE: To optimize Supper Critical CO2 extracting technical (SFE-CO2) methods for extraction of anti-cancer active components of Fig Residues and to investigate the anti-cancer effect of the extract in vitro and in vivo. METHOD: The anti-cancer activity of extracted compound was measured on U937,95D and AGS cancer cells in vitro by MTT method. The anti-cancer effect of the extraction of Fig Residues was studied on mice transplant liver cancer in vivo. RESULT: The SFE-CO2 condition for extraction of the anti-cancer components of Fig Residues was optimized as follows: granularity was 100, the extraction pressure was 30 MPa, the extraction temperature was 45 degrees C, the extraction time was 6 h and the CO2 flux was 12 L x h(-1); The IC50 of anti-cancer active components of Fig Residues on U937, 95D and AGS cells were 70.125 microg x mL(-1), 127.957 microg x mL(-1), 116.000 microg x mL(-1); The anti-cancer active components of Fig Residues inhibited 49.3% of the transplanted liver cancer in the mice. CONCLUSION: The method for extracting the anticancer active components of Fig Residues is stable and reasonable, and the extract from Fig Residues is of the anticancer effect.