G-CSF和IL-6联合应用协同促进受照小鼠造血功能恢复的实验研究

目的 探讨G-CSF和IL-6联合应用协同促进受辐射小鼠造血功能恢复的作用及机理。方法 采用F800血细胞自动计数仪计数红细胞、白细胞、血小板和血红蛋白等指标。常规法进行骨髓集落培养、内源性脾结节计数。琼脂糖电泳方法检测骨髓细胞程序性死亡。结果 G-CSF和IL-6联合应用促进受辐射小鼠外周血细胞的恢复, 促进脾脏内源性脾结节的形成, 增加骨髓细胞含量和促进骨髓细胞GM-CFU的形成, 提高受辐射小鼠的生存率。结论 G-CSF和IL-6联合应用的效果优于单用的效果, 其促进辐射后机体造血功能恢复的机理是通过刺激骨髓残余造血干细胞的恢复, 该过程需要一段较长的时间, 而不是通过抑制照后即刻骨髓细胞的程序性死亡。

Synergistic effect of IL-6 combined with G-CSF on hematological function in irradiated mice

Objective To investigate the synergisitic effects and mechanism of IL-6 combined with GCSF on hematological function in irradiated mice. Methods The numbers of RBC, WBC, PLT and HGB were counted by F-800apparatus.The numbers of CFU-GM and CFUS were counted by usual method.The programmed cell death was assayed by agarose electrophoresis. Results IL-6 combined with GCSF could accelerate the recovery of peripheral blood, promote the recovery of endogenous CFUS, and increase the number of bone marrow cells and stimulate the formation of GM-CFU of bone marrow cells.It could raise survival rate of irradiated mice. Conclusion The synergistic effect of IL-6 combined with GCSF is better than that of either one. The mechanism of the effect is due to accelerative action on the recovery of residual bone marrow stem cells in irradiated mice.This procedure requires long time, and is not attributed to the inhibitory effect upon programmed cell death of bone marrow cells.