不同年龄及退变腰椎间盘中蛋白多糖酶活性测定

目的 测定不同年龄以及退变腰椎间盘中的蛋白多糖酶活性,探讨蛋白多糖酶与年龄、退变之间的关系.方法 对20个腰椎间盘的蛋白多糖酶活性进行测定,其中8个腰椎间盘来自胎儿,6个是退变的成人腰椎间盘,6个是正常的成人腰椎间盘.用含有10mmol/L CaCl2的0.05mol/L Tris/HC对腰椎间盘中的蛋白多糖酶进行提取,用1mmol/L APMA将其激活,与取自牛肩胛软骨的蛋白多糖单体底物进行反应,以酶标仪来测定蛋白多糖酶的活性.结果 胎儿腰椎间盘、退变成人腰椎间盘以及正常成人腰椎间盘的蛋白多糖酶活性的吸光度值分别是1.963±0.487、1.719±0.337、0.870±0.133.胎儿腰椎间盘与正常成人腰椎间盘的吸光度值差异有统计学意义(P<0.01),退变成人椎间盘的吸光度值也高于正常成人椎间盘(P<0.01). 结论腰椎间盘中的蛋白多糖酶活性与年龄、退变相关.

Assay of activity of proteoglycanase in lumbar discs

Objective To assay the activities of proteoglycanases obtained from various lumbar intervertebral discs and explore the relations between proteoglycanase and ages,degneration.Methods The activities of proteoglycanases in 20 spcimens were analyzed.Among these lumbar intervertebral discs,there were 8 fetal lumbar intervertebral discs,6 normal lumbar intervertebral discs and 6 herniated lumbar intervertebral discs.Proteoglycansaes were extracted with 0.05 mmol/L Tris/HCl buffer containing 10 mmol/L CaCl2.The proteoglycan of bovine scapula cartilage extracted with 4.0 mol/L guanidine HCl was used as substrate.The proteoglycan breakdown of the reaction indicated the activity of the proteoglycanases from various luumbar intervertebral discs.Results Activities of proteoglycanases from fetal discs,hernited lumbar intervertebral discs and normal discs were 1.963±0.487,1.719±0.337 and 0.870±0.133 respectively.Activities of proteoglycansaes in fetal discs and hernited discs were greater than normal discs (P<0.01).Conclusion Proteoglycanase plays a important role in the pathological process of degeneration of lumbar intervertebral discs.