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Interpretation of real-time PCR results for hepatitis C virus RNA when viral load is below quantification limits |
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| Authors: | Laperche Syria Bouchardeau Françoise André-Garnier Elisabeth Thibault Vincent Roque-Afonso Anne-Marie Trimoulet Pascale Colimon Ronald Duverlie Gilles Leguillou-Guillemette Hélène Lunel Françoise Bouvier-Alias Magali Pawlotsky Jean-Michel Henquell Cécile Schvoerer Evelyne Stoll-Keller Françoise Chaix Marie-Laure Branger Michel Gaudy-Graffin Catherine Rosenberg Arielle R Pozzetto Bruno Vallet Sophie Baazia Yazid Izopet Jacques Lefrère Jean-Jacques |
| Institution: | Centre National de Référence pour les Hépatites B et C en Transfusion, Département des Agents Transmissibles par le Sang, Institut National de la Transfusion Sanguine, Paris, France. slaperche@ints.fr |
| Abstract: | Hepatitis C virus RNA quantification results obtained in 18 laboratories using real-time PCR methods with 10 negative samples and 22 sample dilutions (viral loads of 0.5 to 500 IU/ml) showed a score of correct results of up to 93.5%. However, 55.6% of the laboratories did not follow the recommendations for the interpretation of their results, leading to ambiguous conclusions. |
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