Inhibition of a soman- and diisopropyl phosphorofluoridate (DFP)-detoxifying enzyme by Mipafox |
| |
| Authors: | F C Hoskin |
| |
| Affiliation: | 1. Key Laboratory of Efficient Utilization of Low and Medium Grade Energy (Tianjin University), Ministry of Education of China, Tianjin, 300350, China;2. Faculty of Engineering, Thamar University, PO Box 87246, Dhamar, Yemen;3. Interdisciplinary Research Center for Renewable Energy and Power Systems, King Fahd University of Petroleum and Minerals, Dhahran, Saudi Arabia;4. Department of Mechanical Power Engineering, Faculty of Engineering, Tanta University, Tanta, 31521, Egypt;1. School of Science, Xi''an Polytechnic University, No.19 of Jinhua South Road, Beilin District, Xi''an, 710048, PR China;2. Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po, New Territories, Hong Kong, 999077, PR China;3. State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi''an, 710072, PR China;4. Department of Physics, School of Pure Science, College of Engineering, Science and Technology, Fiji National University, Lautoka, Fiji;5. College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, PR China;1. College of Horticulture, Gansu Agricultural University, LanZhou, China;2. College of Food Science and Engineering, Gansu Agricultural University, LanZhou, China;3. Institute of Postharvest and Food Sciences, The Volcani Center, Agricultural Research Organization, Rishon LeZion, Israel;1. Department of Bioengineering, Integral University, Lucknow, Uttar Pradesh, India;2. Faculty of Engineering and Technology, Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya, Chitrakoot, Madhya Pradesh, India;3. Department of Biotechnology, Axis Institute of Higher Education, Kanpur, Uttar Pradesh, India;1. Key Laboratory of Biological Resource and Ecological Environment of Chinese Education Ministry, College of Life Sciences, Sichuan University, Chengdu 610064, PR China;2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China;3. Core Laboratory, School of Medicine, Sichuan Provincial People’s Hospital Affiliated to University of Electronic Science and Technology of China, Chengdu 610072, PR China |
| |
| Abstract: | Mipafox, N,N'-diisopropylphosphordiamidofluoridate, has been found to be a reversible competitive inhibitor of a diisopropyl phosphorofluoridate hydrolyzing enzyme (DFPase) isolated from hog kidney and Escherichia coli. Heretofore, this DFPase was characterized by its more rapid hydrolysis of Soman (1,2,2-trimethylpropyl methylphosphonofluoridate), its stimulation by Mn2+, and its wide distribution. In sharp contrast, Mipafox did not inhibit the DFPase found only in cephalopod nerve, hepatopancreas, and saliva, and further characterized by its more rapid hydrolysis of DFP than of Soman, and its indifference to Mn2+. Neither of these two DFPases hydrolyzed Mipafox. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
