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| BMP2、VEGF165双基因共表达质粒在小鼠骨髓基质干细胞的表达 | |
| 引用本文： | 张恒,田晓滨,孙立,杨述华,胡如印,汪雷,陆延盛,张宇坤,傅德皓. BMP2、VEGF165双基因共表达质粒在小鼠骨髓基质干细胞的表达[J]. 贵州医药, 2009, 33(5): 392-395 |
| 作者姓名： | 张恒 田晓滨 孙立 杨述华 胡如印 汪雷 陆延盛 张宇坤 傅德皓 |
| 作者单位： | 1. 遵义医学院 2. 贵州省人民医院骨科,550002 3. 华中科技大学同济医学院附属协和医院骨科,武汉,430022 |
| 基金项目： | 贵州省科学技术基金，贵州省优秀科技教育人才省长专项基金 |
| 摘 要： | 目的观察转染BMP2和VEGF165双基因共表达质粒在小鼠骨髓基质干细胞的表达情况。方法脂质体介导下将双基因真核表达质粒pIRES-BMP2-VEGF165导入小鼠骨髓基质干细胞，用RT-PCR和免疫组织化学方法观察BMP2和VEGF165双基因在小鼠骨髓基质干细胞内的表达。结果转染BMP2、VEGF165的小鼠骨髓基质干细胞有明显的BMP2和VEGF165 mRNA及其蛋白表达。结论转染BMP2和VEGF165双基因的小鼠骨，髓基质干细胞能同时表达以上两种基因。 |
| 关 键 词： | 骨形成蛋白2 血管内皮生长因子165 骨髓基质干细胞 |
Expression of BMP2 and vegf165 genes simultaneity transfected mouse bone marrow mesenchymal stem cells |
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| Zhang Heng,Tian Xiaobing,Sun Li,Yang Shuhua,Hu Ruying,Wang Lei,Lu Yanshen,Zhang Yukun,Fu Dehao. Expression of BMP2 and vegf165 genes simultaneity transfected mouse bone marrow mesenchymal stem cells[J]. Guizhou Medical Journal, 2009, 33(5): 392-395 | |
| Authors: | Zhang Heng Tian Xiaobing Sun Li Yang Shuhua Hu Ruying Wang Lei Lu Yanshen Zhang Yukun Fu Dehao |
| Affiliation: | Zhang Heng , Tian Xiaobing, Tian Xiaobing, Sun Li, Yang Shuhua, Hu Ruying, Wang Lei , Lu Yanshen ,Zhang Yukun, Fu Dehao ( 1. ZunyiMedical College,Zunyi, 563003, Guizhou, China; 2. Department of Orthopedics, Guizhou Provincial People＇s Hospital, Gui yang , 550002, Guizhou, China; 3. Department of Orthopedics, The Affiliated Hospital of Tongji Medical College Huazong Scientific and Technological University ,Wuhan, 430022, Hubei, China) |
| Abstract: | Objective To evaluate the simultaneity expression efficacy of mouse bone marrow stromal cells （mMSCs） transfected with bone morphogenetic protein 2 （BMP2） and vascular endothelial growth factor 165 （VEGF165） genes. Methods The mMSCs were transfected with pIRES-BMP2- VEGF165 by liposome-mediated method. The co-expression of BMP2 and VEGF165 genes were determined by RT-PCR and immunohistochemical analysis. ResultsRT-PCR and immunohistochemical analysis showed that these transfected cells indeed could produce the mRNA and proteins of the BMP2 and VEGF165 ex vivo and in vivo. Conclusion The mMSCs transfected with BMP2 and VEGF165 ：genes will be expression simultaneously of mouse marrow stromal cells. |
| Keywords: | Bone morphogenetic protein2 Vascular endothelial factor 165 Bone marrow Mesenchymal stem cells |
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