凉血化瘀中药与曲安奈德对脉络膜新生血管动物模型中MMP-9及TIMP-2表达的影响

目的:评价凉血化瘀中药及曲安奈德对氪激光诱导BN大鼠脉络膜新生血管(choroidal neovascularization CNV)中基质金属蛋白酶MMP-9和基质金属蛋白酶抑制剂TIMP-2表达的影响。方法:659nm激光诱导BN大鼠CNV模型,随机分成空白对照组(每日生理盐水灌胃)、中药组(每日中药灌胃)和曲安奈德组(激光后1d玻璃体腔注射曲安奈德5μL,0.2mg)各16只。分别在光凝后7,14,21,28d随机选取4只大鼠处死,摘除眼球,行病理切片及免疫组织化学检测MMP-9及TIMP-2蛋白在CNV组织中的表达。结果:空白对照组在光凝后7dMMP-9达到高峰,此后逐渐下降;中药组在光凝后14d达到高峰,但峰值低于空白对照组,此后逐渐下降;曲安奈德组在光凝后7d达到高峰,峰值介于空白对照组和中药组之间,此后逐渐下降,在14～21d出现平台期。空白对照组在光凝后14dTIMP-2达到高峰,此后逐渐下降;中药组在光凝后14d达到高峰,峰值高于空白对照组,此后逐渐下降;曲安奈德组在光凝后14d达到高峰,峰值介于空白对照组和中药组之间,此后下降。结论:在下调MMP-9、上调TIMP-2的表达进而抑制CNV形成方面,凉血化瘀中药的作用强于曲安奈德。

Expression of MMP-9 and TIMP-2 in experimental choroidal neovascularization utiliz-ing liangxuehuayu decoction and triamcinolone acetonide

AIM:To evaluate the changes of MMP-9 and TIMP-2 in rat model of choroidal neovascularization(CNV)taking liangxuehuayu decoction and triamcinolone acetonide (TA). ·METHODS: Both eyes of 48 rats received a series of 20 spots of laser irradiation (659nm, 360mW, 50μm, 0.05s). The rats were divided into three groups: the control group (received 9g/L physiological saline by gastric gavage, 16 rats), the TCM group (liangxuehuayu decoction drugs by gastric gavage, 16 rats) and TA group (intravitreous injection with TA 5μL, 0.2mg, 16 rats). Histopathologic examination(HE), MMP-9 and TIMP-2 straining by immunohistochemistry were performed on 7,14,21 and 28 days after photocoagulation.·RESULTS: The staining intensity of MMP-9 in CNV showed that in control group the staining intensity was reaching the peak on day 7, and then decreased greatly. In TCM group the staining intensity was reaching the peak on day 14, the peak was lower than the control group and then decreased greatly. In TA group the staining intensity was reaching the peak on day 7, but the degree was between the control group and TCM group, then decreased. A platform stage occur on day 14-21. The TIMP-2’s staining intensity showed that in control group the staining intensity was reaching the peak on day 14, and then decreased greatly. In TCM group the staining intensity was reaching the peak on day 14, but the peak was higher than the control group and then decreased greatly. In TA group the staining intensity was reaching the peak on day 14, but the degree was between the con-trol group and the TCM group, then decreased greatly.·CONCLUSION: The liangxuehuayu decoction and TA can also suppress experimental CNV induced by Krypton laser through the regulation of MMP-9 and TIMP-2, and the liangxuehuayu decoction has a stronger effect to regulate the system of MMPs-TIMPs than TA.