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| 血管平滑肌细胞不同细胞周期中Mfn2的表达变化 | |
| 引用本文： | 靳国青,周炜,黄晴,糜涛,曹文静,陈莉莉,郭小梅.血管平滑肌细胞不同细胞周期中Mfn2的表达变化[J].广东医学,2010,31(9). |
| 作者姓名： | 靳国青 周炜 黄晴 糜涛 曹文静 陈莉莉 郭小梅 |
| 作者单位： | 1. 华中科技大学同济医学院附属同济医院心血管内科,武汉,430030 2. 华中科技大学同济医学院附属同济医院综合科,武汉,430030 3. 华中科技大学同济医学院附属同济医院检验科,武汉,430030 |
| 摘 要： | 目的 探讨血管平滑肌细胞不同细胞周期中线粒体融合蛋白 2(mitofusin 2,Mfn2)表达水平的变化.方法 通过血清饥饿法、胸腺嘧啶核苷(thymidine)和诺考达唑(nocodazole)干预,使血管平滑肌同步化在不同的细胞周期(G0/G1期、G1/S期、S期、G2/M期);流式细胞仪鉴定细胞周期;Western blot检测Mfn2在不同细胞周期中的表达水平.结果 (1)流式细胞仪鉴定各组DNA含量百分比显示G0/G1期、G1/S期、S期、G2/M期均已同步化,符合本次实验要求.(2)Western blot示:同步化干预后,Mfn2在G0/G1期(静止期)的表达量显著高于G1/S期及S期(增殖期);自然状态下,Mfn2在G0/G1期表达量亦明显高于S期.结论 Mfn2表达量随细胞周期的变化而变化,Mfn2在细胞周期的静止期的表达量明显高于增殖期. |
| 关 键 词： | 线粒体融合蛋白2 血管平滑肌细胞 细胞周期 |
Mfn2 expression in the different phases of cell cycle of rat aortic smooth muscle cells |
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| JIN Guo-qing,ZHOU Wei,HUANG Qing,CAO Wen-jin,CHEN Li-li,GUO Xiao-mei.Mfn2 expression in the different phases of cell cycle of rat aortic smooth muscle cells[J].Guangdong Medical Journal,2010,31(9). | |
| Authors: | JIN Guo-qing ZHOU Wei HUANG Qing CAO Wen-jin CHEN Li-li GUO Xiao-mei |
| Institution: | JIN Guo-qing,ZHOU Wei,HUANG Qing,CAO Wen-jin,CHEN Li-li,GUO Xiao-mei. Department of Cardiology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
| Abstract: | Objective To evaluate the change of mitofusin 2(Mfn2) expression in the different phases of cell cycle of rat thoracic aortic smooth muscle cells (SMC) in vitro. Methods Serum starvation and drug intervention were introduced to synchronize cells in the different phases of cell cycle (G0/G1-phase,G1/S-phase,S-phase,G2/M-phase) and flow cytometry was used for identification. The expression of Mfn2 was detected by Western blot. Results (1) Flow cytometry revealed that cells were synchronized in G0/G1-phase,G1/... |
| Keywords: | mitofusin 2 vascular smooth muscle cell cell cycle |
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