非小细胞肺癌组织中NF-kB和c-myc及p73的表达与意义

目的：探讨NF-κB（p65）、c-myc和p73蛋白在非小细胞肺癌（non-small cell lung cancer，NSCLC）组织中的表达及相互关系。方法：用免疫组化二步法检测60例NSCLC组织标本中p65、c-myc和p73蛋白的表达，并进行相关分析。结果：p65、c-myc和p73蛋白在肺癌组织中的表达分别与在肺支气管上皮组织的表达比较差异有统计学意义，P〈0．05。p65和c-myc蛋白在淋巴结转移癌组织中的表达与肺支气管上皮组织中的表达差异无统计学意义，P〉0．05；p73蛋白在上述两组的表达差异有统计学意义，P〈0．05。p65、c-myc和p73蛋白在肺癌高、中与低分化阳性表达的比较，差异均无统计学意义，P〉0．05。伴有淋巴结转移的肺癌组织中蛋白阳性表达与无淋巴结转移比较：p65和c-myc差异无统计学意义，P〉0．05；p73差异有统计学意义，P〈0．05。肺癌组织中p65与c-myc具有相关性，R=0．5514，P〈0．01；c-myc与p73具有相关性，R=0．6537，P〈0．01；未发现p65与p73有明显相关性，R=0．1735，P〉0．05。结论：p65、c-myc和p73表达上调均可能是NSCLC发生的重要因素；在肺癌发生发展中p65与c-myc、c-myc和p73可能在诱导细胞增生与抑制细胞凋亡中起协同作用；p73上调可能促进肺癌的侵袭与转移，可作为肺癌恶化进展及癌转移的动态监控标志。

Expressions and implications of p65,c-myc and p73 proteins in non-small cell lung cancer

OBJECTIVE: To study the expressions of p65, c-myc and p73 proteins in non-small cell lung cancer (NSCLC) and their clinical implications. METHODS: In 40 cases of NSCLC, two-step immunohistochemistry was used to detect the expressions of p65, c-myc and p73 proteins in tissue samples. RESULTS: It had a significant difference that p65,c-myc and p73 proteins expressed positive between the lung cancer tissues and the normal bronchial epithedium, P<0.05; p65 and c-myc proteins had no significant difference between the lymph node metastasis of lung cancer and the normal bronchial epithedium, but p73 protein had a significant difference between the two comparative tissues, P<0.05. The positive expressions of p65, c-myc and p73 proteins had no significant difference in high differentiation degree and medium differentiation degree compared with low differentiation degree in lung cancer tissues, P>0.05. The expressions of p65 and c-myc proteins in lung cancer had no significant difference between the lung cancer tissues with transferred of lymph node and ones without transferred lymph node (P>0.05), but the expression of p73 proteins had a significant difference between the two comparative tissues, P<0.05. In lung cancer, p65 was positively correlated with c-myc, r=0.551 4, P<0.01; c-myc was positively correlated with p73, r=0.653 7, P<0.01; p65 was not correlated with p73, r=0.173 5, P>0.05. CONCLUSIONS: The expressions of p65 c-myc and p73 proteins gone up may be the abnormity of cancer gene in NSCLC. p65 is positively correlated with c-myc in generation and progress of lung cancer. While c-myc is positively correlated with p73, it may draw a conclusion that the two genes can induce cell hyperplasia and restrain cell apoptosis. The expression of p73 protein gone up in the lymph node metastasis and lung cancer with transfer of lymph node, may deduce that this gene facilitates the soakage and metastasis of lung cancer. It would be a sign of the worsening and prognosis in lung cancer.