天冬胶作为血管栓塞剂毒性实验的初步报告

目的:从其毒性和相关特性方面研究天冬胶作为血管栓塞剂的可行性。方法:昆明小鼠腹腔注射天冬胶后观察外貌行为、食物消耗、体重情况。最后检测WBC、血小板、RBC和Hb浓度;测定血清ALT、肌酐和尿素氮浓度。病理检查心、肝、肾、脾、脑、腹膜、生精组织及腹壁肌肉。大腿肌肉注射天冬胶的小鼠1、3、7 d后各处死,取局部肌肉标本送病理检查。结果:1.仅实验组给药后第1天反应稍差及食量显著低于对照组(P<0.001)外,第2天起各组外貌行为无异常、食量无显著差异(均P>0.05)。2.各组小鼠的体重随时间而增加,除实验组2于实验1周后增长速度显著低于实验组1(P<0.02)和对照组(P<0.001)外,其他无显著差异。3.各组血WBC、血小板、RBC、Hb及血清ALT、肌酐和尿素氮浓度无显著差别。4.仅1个实验组小鼠肝脏内可见多灶性淋巴细胞和其他炎症细胞浸润区,其他组肝脏表现正常。实验组腹膜出现少许慢性炎症细胞的浸润和间皮细胞的增生。其他均无病理改变。大腿肌肉内仅见少许残留异物,无病理改变。结论:天冬胶使用安全、生物相容性较好,作为血管栓塞剂有较好的前景。

Study on toxicity of asparagus colloid as vascular embolization agent-preliminary experience

Objective: To explore the feasibility of asparagus colloid as vascular embolization agent. Methods: The appearance, behavior and weight of the Kunming' s mouses were monitored after they were administrated (i. p.) asparagus colloid,. The numbers of white blood cell (WBC) , platelet, red blood cell (RBC) and hemoglobin (Hb) , serum ALT, creatinine and urine nitrogen were examined. The heart, liver, kidney, spleen, brain, peritoneum, sepermatogenic tissue and muscle of abdominal wall were examined pathologically after the mouses were sacrificed. Asparagus colloid (0.1 ml) was injected into posterior thigh of 12 mouses and the local muscle was examined pathologically within 1,3,7 days.Result: 1. The reaction was slightly unconcerned and the consume of food decreased significantly ( P <0.00l) in the experimental groups only in the first day after the injection compared with control group. The rest were normal and had no significant difference ( P >0.05) in all groups. 2. The weight of mouse in all groups increased with time. The rate of weight increase had no difference in all groups, except in group 2 which was significantly less than that in group 1( P < 0.02) and control group ( P < 0.001) . 3. The numbers of WBC, platelet and RBC, Hb, serum ALT, creatinine and urine nitrogen had no significant difference ( P > 0.05) in all groups. 4. There were some nodules composed of lymphocyte and other inflammation cells in the liver only in group 1 , however in other groups the liver was normal. There was infiltration of some inflammation cells and proliferative mesothelial cells in the peritoneum in group 1 and 2. Other organs and tissues were normal in all groups. There were a little residual foreign body in muscle of posterior thigh, in which there was no antixenic reaction and pathological change. Conclusion: As a safe and good biologic compatibility, Asparagus colloid whould have a bright future as a potential angioembolic agent.