| 流行性出血热病毒血凝和血凝抑制试验的改进 |
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| 引用本文： | 欧阳学农 倪大石. 流行性出血热病毒血凝和血凝抑制试验的改进[J]. 中国人兽共患病杂志, 1990, 6(6): 36-38 |
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| 作者姓名： | 欧阳学农 倪大石 |
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| 作者单位： | 第四军医大学二院传染科快诊室，第四军医大学二院传染科快诊室，安徽省医学科学研究所病毒室 南京军区福州总医院二部传染科 |
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| 摘 要： | 本文对流行性出血热研究和诊断中传统采用的血凝和血凝抑制试验进行较大改进。比较7种制备流行性出血热病毒血凝素的方法,首次推出制备较高滴度血凝素的吐温80-乙醚法;试用含CaCl_2和MRSO_4 各0.05％的pH9.0 BABS稀释血凝素,血凝滴度较仅用pH9.0 BABS稀释者高4 倍;测定6种动物血球对血凝素的敏感性,找到了比鹅血球更敏感的鸽血球;采用以二甲亚砜为主要成份的血球冻存液,将鸽血球保存于-20℃,大大延长了血球使用期限而不影响其质量。
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| 关 键 词： | 流行性出血热 病毒 血凝 抑制试验 |
Improred Medium for Hemagglutination and Hemagglutination-inhibition Test of EHFV |
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| Ou Yang Xuenong. Improred Medium for Hemagglutination and Hemagglutination-inhibition Test of EHFV[J]. Chinese Journal of Zoonoses, 1990, 6(6): 36-38 |
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| Authors: | Ou Yang Xuenong |
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| Affiliation: | Ou Yang Xuenong |
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| Abstract: | In this report,we have modified the HAT-HIT for diagnosis of EHF. EHFV-HAN was made by 7 methods,the highest HA titer of EHFV-HAN was obtained by Tween 80-ether method. The pH 9. 0 BABS containing 0. 05% CaCl2and MgSO4was more suitable for being used as the dilution of EHFV-HAN than pH 9. 0 BABS. Pigeon erythrocytes were the most sensitive to EHFV-HAN among 6 sorts of red blood cells. Frozen pigeon erythrocytes were used instead of fresh goose erythrocytes and fresh pigeon erythrocytes. The HI titers obtained with frozen pigeon erythrocytes were no less than those obtained with fresh erythrocytes. It concluded that with the modified methodology as shown in our study,HAT and HIT will be well appropriate for routine laboratory. |
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| Keywords: | EHFV HAT HIT. |
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