| Ca2+信号通路对肝癌细胞乙酰肝素酶表达的影响 |
| |
| 引用本文： | 周少英,王顺祥,吴晓慧,李建坤,杨宝明,冯宁宁,张越山.Ca2+信号通路对肝癌细胞乙酰肝素酶表达的影响[J].现代中西医结合杂志,2011,20(16):1957-1959. |
| |
| 作者姓名： | 周少英 王顺祥 吴晓慧 李建坤 杨宝明 冯宁宁 张越山 |
| |
| 作者单位： | 1. 河北省邯郸市中心医院,河北,邯郸,056001
2. 河北医科大学第四医院,河北,石家庄,050011 |
| |
| 摘 要： | 目的探讨Ca2+信号调节通路对肝癌细胞乙酰肝素酶的表达及分泌的影响。方法不同浓度的Ca2+及其激动剂毒胡萝卜内酯(TG)和抑制剂S-亚硝基-乙酰青霉胺(SNAP)作用于SMMC-7721细胞,RT-PCR检测肝癌细胞乙酰肝素酶mRNA表达的变化;W estern-b lot检测肝癌细胞及其培养上清乙酰肝素酶蛋白表达的变化。结果 Ca2+对肝癌细胞乙酰肝素酶mRNA的表达没有影响,随Ca2+浓度的增加,细胞培养上清和肝癌细胞胞浆乙酰肝素酶蛋白表达增加;Ca2+激动剂TG能诱导肝癌细胞内及培养上清中HPA蛋白表达,这种诱导作用能被TG抑制剂SNAP抑制。结论 Ca2+通过细胞内NO→cGMP调控的Ca2+信号通路促进肝癌细胞内乙酰肝素酶蛋白表达和分泌。
|
| 关 键 词： | 乙酰肝素酶 Ca2+信号通路 肝癌 |
Effect of Ca2+ signaling pathway on heparanase expression of human liver cancer cell lines |
| |
| Zhou Shaoying,Wang Shunxiang,Wu Xiaohui,Li Jiankun,Yang Baoming,Feng Ningning,Zhang Yueshan.Effect of Ca2+ signaling pathway on heparanase expression of human liver cancer cell lines[J].Modern Journal of Integrated Chinese Traditional and Western Medicine,2011,20(16):1957-1959. |
| |
| Authors: | Zhou Shaoying Wang Shunxiang Wu Xiaohui Li Jiankun Yang Baoming Feng Ningning Zhang Yueshan |
| |
| Institution: | Zhou Shaoying1,Wang Shunxiang2,Wu Xiaohui2,Li Jiankun2,Yang Baoming2,Feng Ningning2,Zhang Yueshan2(1.The Central Hospital of Handan City,Handan 050011,Hebei,China,2.The Forth Hospital of Hebei Medical University,Shijiazhuang 050011,China) |
| |
| Abstract: | Objective It is to investigate the effect of Ca2+ signal on heparanase expression and secretion of human liver cancer cell line SMMC-7721 in vitro.Methods SMMC-7721 cells were cultured with Ca2+ and its xcitomotor Thapsigargin(TG) or inhibitor S-nitro-N-acetylpenicillamine(SNAP) with various concentrations.All of the cells were collected on time.Heparanase mRNA expression was detected using RT-PCR and heparanase protein expression detected using Western blot assay respectively.Results Ca2+ had no effect on ... |
| |
| Keywords: | heparanase Ca2 signaling pathway hepatocarcinoma |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
